Surface properties can affect cell attachment and growth ability on the biomaterials. In this work, the effect of the morphology and chemical composition of the surface coatings synthesized on zirconium (Zr) substrates on bioactivity was studied. The surface of pure Zr was modified via a plasma electrolytic oxidation process to obtain oxide-based coatings with different surface chemical compositions and pore sizes by changing the process parameters in the same electrolyte. The characterization of the coatings was conducted using X-ray diffraction (XRD), scanning electron microscopy (SEM), contact angle goniometry (CAG) and energy dispersive spectroscopy (EDS), and Fourier transform infrared spectroscopy (FTIR). The results showed that the coatings comprised t-ZrO₂ and Ca_0.134Zr_0.86O_1.86 phases. During the oxidation of Zr substrates, applying an anode current with different densities and frequencies altered the surface roughness Ra of the samples. The relationship between the surface morphology, chemical composition, and bioproperties was investigated by simulated body fluid (SBF) and cell culture tests. The coated samples were immersed into SBF solution and the formation of hydroxyapatite (HA) structure on the surface was detected after 7 days of immersion. A close relationship between the development of HA, the size of the porosity and chemical composition of the coating surface was observed. Additionally, the cell attachment and cell growth on the surface were found to be dependent on the chemistry and topography of coated surfaces. The cell culture test revealed that the growth of the cells was improved with increasing pore sizes on the coating surface.

表面特性会影响细胞在生物材料上的附着和生长能力。在这项工作中，研究了锆（Zr）基底上合成的表面涂层的形貌和化学组成对生物活性的影响。通过等离子电解氧化工艺对纯Zr的表面进行改性，以通过改变同一电解液中的工艺参数来获得具有不同表面化学成分和孔径的氧化物基涂层。使用X射线衍射（XRD），扫描电子显微镜（SEM），接触角测角法（CAG）和能量色散光谱（EDS）以及傅里叶变换红外光谱（FTIR）对涂层进行表征。结果表明，该涂层包含t-ZrO ₂和Ca _0.134 Zr_0.86 O _1.86相。在Zr衬底氧化过程中，施加不同密度和频率的阳极电流会改变表面粗糙度Ra样本。通过模拟体液（SBF）和细胞培养试验研究了表面形态，化学成分和生物特性之间的关系。将涂覆的样品浸入SBF溶液中，浸入7天后检测表面上羟基磷灰石（HA）结构的形成。观察到HA的发展，孔隙大小与涂层表面化学组成之间的密切关系。另外，发现表面上的细胞附着和细胞生长取决于涂覆表面的化学性质和形貌。细胞培养测试表明，随着涂层表面孔径的增加，细胞的生长得到改善。