We determined that T-cell astrocyte interaction modulates interleukin-10 (IL-10) production from both cell types. The impact of IL-10 on astrocytes was compared to IL-10 generated from T-cell-astrocyte interactions in vitro. We demonstrated that T-cells directly interact with astrocytes to upregulate gene expression and secretion of IL-10, confirmed by elevated STAT3p/STAT3 expression in astrocytes. IL-10 increased astrocytes proliferation. In addition, IL-10 treatment and CD4+ co-culture shifts primary astrocytes toward a more energetic phenotype. These findings indicate that direct interaction of CD4+ T-cells with astrocytes, activated the IL-10 anti-inflammatory pathway, altering astrocyte phenotype, metabolism, and proliferation.

中文翻译：

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星形胶质细胞表型对 T 细胞相互作用的调节

我们确定 T 细胞星形胶质细胞相互作用调节两种细胞类型的白细胞介素 10 (IL-10) 的产生。将 IL-10 对星形胶质细胞的影响与体外 T 细胞-星形胶质细胞相互作用产生的 IL-10 进行比较。我们证明了 T 细胞直接与星形胶质细胞相互作用以上调 IL-10 的基因表达和分泌，这通过星形胶质细胞中 STAT3p/STAT3 表达升高得到证实。IL-10 增加星形胶质细胞增殖。此外，IL-10 治疗和 CD4+ 共培养使原代星形胶质细胞向更有活力的表型转变。这些发现表明，CD4+ T 细胞与星形胶质细胞的直接相互作用，激活了 IL-10 抗炎途径，改变了星形胶质细胞的表型、代谢和增殖。