A systematic methodology was used to quantify ganoderic acid-A (GA-A) loaded nano-lipid carriers (NLC) in rat plasma using UPLC-MS/MS. Separation of the analyte was achieved using ACQUITY UPLC BEH C₁₈ column (1.7 µm) and mobile phase as water containing 0.1% Acetonitrile (40: 60% v/v) at a flow rate of 0.4 mL·min⁻¹. The analyte was detected using MRM mode to track precursor-to-product ion transitions of 515.37 → 285.31 m/z (time scan of 2 min) for GA-A, and 175.11 → 115.08 m/z (time scan of 4 min) for ascorbic acid as an internal standard (IS), respectively. The developed method was validated for linearity, accuracy, within and between day precisions, limit of quantification and recovery of the analyte. The results indicated intra and inter-day consistency and precision values were found to be within the acceptance limit for the plasma samples. The method applicability for determination of pharmacokinetic parameters of GA-A was assessed after oral administration of free GA-A solution and GA-A-loaded NLC, which indicated significant difference (p < 0.05) in the rate and extent of absorption parameters of GA-A from the NLC formulation vis-à-vis the plain solution. Overall, the studies construed successful development and application of UPLC-MS/MS method for estimation of GA-A in the lipidic formulation.

使用系统的方法，使用UPLC-MS / MS定量测定大鼠血浆中载有灵芝酸A（GA-A）的纳米脂质载体（NLC）。使用ACQUITY UPLC BEH C ₁₈色谱柱（1.7 µm）和流动相以0.1 mL乙腈（40：60％v / v）的水以0.4 mL·min ^-1的流速进行分离。使用MRM模式检测分析物，以跟踪GA-A的前体离子到产物离子的跃迁，即515.37→285.31  m / z（2分钟的时间扫描），以及175.11→115.08  m / z（4分钟时间扫描）分别将抗坏血酸作为内标（IS）。验证了所开发方法的线性，准确性，日内和日间精度，定量限和分析物的回收率。结果表明日间和日间一致性和精确度值在血浆样品的可接受极限内。口服游离GA-A溶液和载有GA-A的NLC评估口服GA-A药代动力学参数的方法适用性，表明GA吸收参数的速率和程度存在显着差异（p <0.05） NLC配方相对于普通溶液的-A。总体而言，研究表明成功开发了UPLC-MS / MS方法用于脂质制剂中GA-A的估算。