Recent years have witnessed the growing popularity of deep eutectic solvents (DESs) in the extraction of functional components. As a novel green alternative to organic reagents, DESs are environmentally friendly, non-toxic, inexpensive, and biodegradable. In the present study, tailor-made DESs were prepared and applied for the first time to extract flavonoids from rhizomes of Polygonatum odoratum. We also investigated the extraction process, extraction efficiency, and solvent recovery efficiency. The response surface methodology (RSM) was adopted to optimise total flavonoid extraction, and the DES was recovered through macroporous resin adsorption. The results show that the DES synthesised by choline chloride and lactic acid at a molar ratio of 1:2 was the most suitable extraction medium. Extraction conditions were optimised as follows: extraction temperature of 51 °C, DES water content of 27%, liquid–solid ratio of 22 mL/g, and extraction time of 21 min. Under these conditions, the extraction rate of total flavonoids reached 11.47 mg/g, close to that of the heat reflux extraction (HRE) with organic solvents. The adsorption and desorption experiments of flavonoids on three types of macroporous resins (HPD826, D101, and AB8) were carried out, and D101 showed higher adsorption/desorption capacities, successively followed by AB8 and HPD826. The extraction rate of the recycled DES reached 10.78 mg/g, putting the reuse rate at 93.98%. In conclusion, the developed DES combined with macroporous resin enrichment can be an alternative method for green and efficient extraction of bioactive flavonoids from plant materials.

近年来，深层共晶溶剂（DES）在功能成分的提取中日益普及。作为有机试剂的新型绿色替代品，DES是环保，无毒，廉价且可生物降解的。在本研究中，制备了量身定制的DES，并首次将其用于从玉竹的根茎中提取黄酮类化合物。。我们还研究了萃取过程，萃取效率和溶剂回收效率。采用响应面法（RSM）优化总黄酮提取，并通过大孔树脂吸附回收DES。结果表明，氯化胆碱和乳酸以1：2的摩尔比合成的DES是最合适的提取介质。萃取条件的优化如下：萃取温度为51°C，DES水含量为27％，液固比为22 mL / g，萃取时间为21分钟。在这些条件下，总黄酮的提取率达到11.47 mg / g，接近于用有机溶剂进行热回流提取（HRE）的提取率。黄酮类化合物在三种大孔树脂（HPD826，D101，和AB8），D101表现出更高的吸附/解吸能力，其次是AB8和HPD826。回收的DES的提取率达到10.78 mg / g，重复使用率达到93.98％。总之，开发的DES与大孔树脂富集相结合可以成为从植物材料中绿色高效提取生物活性类黄酮的另一种方法。