Abstract

This study was carried out to determine the main pigments in some different selected seaweeds and to reveal their antioxidant potential regarding the ever-increasing demand for utilization of marine pigments in human health and nutrition. The individual amounts of algal pigments were found by reverse phase high-performance liquid chromatography (HPLC) and their total antioxidant capacities (TAC) by two spectrophotometric TAC assays, namely: CUPRAC (CUPric ion Reducing Antioxidant Capacity) and ABTS/TEAC (2,2′-azinobis [3-ethyl benzo thiazoline-6-sulfonate])/(trolox equivalent antioxidant capacity). These two tests gave the same rank order for TAC. The TAC of HPLC-quantified compounds accounted for a relatively much lower percentage of the observed CUPRAC capacities of seaweed extracts, namely ranging from 11 to 68% for brown, from 4 to 41% for red and from 3 to 100% for green species, i.e., some TAC originated from chromatographically unidentified compounds. Fucoxanthin, chlorophyll a, and pheophytin a compounds were major pigments in brown algae. The relative carotenoid contents in red marine algae were generally lower than those of chlorophylls. Overall total quantities were quite low compared with those of brown species. In general, chlorophyll a and chlorophyll b were chiefly present in green algae, but β-carotene, violaxanthin and siphonaxanthin were also detected substantially higher in some species of green algae such as Caulerpa racemosa var. cylindracea and Codium fragile.

中文翻译：

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高效液相色谱检测和海藻提取物中棕色，红色和绿色藻类色素的抗氧化能力测定

抽象的

进行这项研究是为了确定一些不同的选定海藻中的主要色素，并揭示其在人类健康和营养中利用海洋色素的需求不断增长的抗氧化潜力。通过反相高效液相色谱（HPLC）和两种分光光度法TAC测定法分别测定了藻类色素的单独含量和总抗氧化能力（TAC）：CUPRAC（降低CUPric离子的抗氧化能力）和ABTS / TEAC（2， 2′-氮杂双[3-乙基苯并噻唑啉-6-磺酸盐]）/（trolox当量抗氧化剂容量）。这两个测试给出了TAC相同的等级顺序。HPLC定量化合物的TAC占所观察到的海藻提取物CUPRAC容量的百分比相对较低，即棕色占11％至68％，红色占4％至41％，绿色物种占3％至100％，即某些TAC来源于色谱未鉴定的化合物。岩藻黄质黄嘌呤a和ophophytin a化合物是褐藻中的主要色素。红海藻中的相对类胡萝卜素含量通常低于叶绿素。与棕色物种相比，总体总量非常低。通常，绿藻中主要存在叶绿素a和叶绿素b，但是在某些绿藻物种中，例如菜豆（Caulerpa racemosa var），β-胡萝卜素，紫黄质和虹吸黄质的含量也显着较高。圆柱状和脆弱的dium。