Abstract

An appropriate purification and quantification method has been developed for polycyclic aromatic hydrocarbons (PAH) in hydro alcoholic herbal extracts. For this, Bacopa monnieri, Camellia sinensis, Withania somnifera and Andrographis paniculata samples were extracted with modified solid-phase extraction (SPE) and the PAH were quantified using liquid chromatography coupled to fluorescence detector. Purification of herbal extract using hexane and acetone in the ratio of 1:1 followed by treatment with QuEChERS salt (6 g MgSO4 and 1.5 g sodium acetate) improved the recovery rate of PAH. Silica SPE, which accomplishes solvent exchange to hexane by cleanup method, was developed to reduce the matrix effect and quality of the result obtained was increased. The developed method can be used for regular monitoring and analysis of PAH in natural extracts so as to prevent contamination.

中文翻译：

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固相萃取-荧光检测器测定水乙醇提取物中的PAH苯并[蒽]，苯，苯[[b]丁二烯]和苯并[a] py

摘要

已经开发了一种适当的纯化和定量方法，用于对含氢草药提取物中的多环芳烃（PAH）进行分析。为此，Bacopa monnieri，山茶，Withania somnifera和穿心莲样品采用改进的固相萃取（SPE）萃取，并使用液相色谱仪和荧光检测器对PAH进行定量。使用己烷和丙酮以1：1的比例纯化草药提取物，然后用QuEChERS盐（6 g MgSO4和1.5 g乙酸钠）处理可提高PAH的回收率。开发了通过净化方法完成溶剂交换成己烷的硅胶SPE，以降低基质效应，并提高所得结果的质量。所开发的方法可用于定期监测和分析天然提取物中的PAH，以防止污染。