Abstract

Alpha-linolenic acid (ALA) and linoleic acid (LA), abundant in chia seed oil, are useful polyunsaturated fatty acids (PUFA) with numerous health benefits. The objectives of the present study were to explore the optimum extraction condition of chia seed oil and the possibilities of direct analysis of ALA and LA in chia seed oil by reversed-phase high-performance liquid chromatography with ultraviolet detection (RP-HPLC-UV). The optimized chia seed oil extraction condition was set by the usage of Soxhlet extrator with hexane as a solvent, with the solvent to solid ratio of 8 and the extraction time of 8 h. Prior to HPLC-UV analysis, the oil was saponified in order to get the free fatty acids for detection. The results showed that the proposed HPLC-UV method allowed the quantification of ALA and LA in chia seed oil. The method was simple, rapid (within 18 min) and sensitive (limit of detection 0.006 mg/mL for ALA and 0.02 mg/mL for LA) and precise (RSD ≤ 2%). Thus, the proposed experimental designs were shown to offer considerable advantages over traditional derivatization approaches in the ALA and LA analyses.

中文翻译：

---

高效液相色谱法定量分析奇亚籽油的多不饱和脂肪酸

摘要

奇亚籽油中富含的α-亚麻酸（ALA）和亚油酸（LA）是有用的多不饱和脂肪酸（PUFA），具有许多健康益处。本研究的目的是探索通过反相高效液相色谱-紫外检测（RP-HPLC-UV）来研究正大籽油的最佳提取条件以及直接分析正大籽油中的ALA和LA的可能性。 。以己烷为溶剂的索氏萃取剂设定了奇异籽油的最佳提取条件，溶剂固比为8，提取时间为8 h。在进行HPLC-UV分析之前，先将油皂化以得到游离脂肪酸进行检测。结果表明，所提出的HPLC-UV方法可以定量分析正大籽油中的ALA和LA。方法很简单 快速（18分钟内）和灵敏（ALA的检出限为0.006 mg / mL，LA的检出限为0.02 mg / mL）和精确的（RSD≤2％）。因此，在ALA和LA分析中，拟议的实验设计显示出比传统衍生化方法可观的优势。