A novel, sensitive and rapid high performance liquid chromatography (HPLC) method for the determination of ceftiofur by pre-column derivatization with 1,2-naphthoquinone-4-sulfonate. Analysis was performed within 5 min on a Kinetex C18 column based on core-shell technology. The mobile phase composed of acetonitrile-water (50:50, v/v) pumped isocratically at a flow rate of 1.0 mL/min under UV detection at 254 nm. The factors affecting the derivatization reaction and separation conditions were carefully evaluated and optimized. The method was linear over the concentration range of 45–450 ng/mL with a limit of detection of 3.29 ng/mL and limit of quantitation of 10.97 ng/mL. The new method was successfully applied for the analysis of ceftiofur in the veterinary formulation and honey with average recoveries of 100.78% and 98. 83%, respectively. The present method is suitable and favorable for the analysis of ceftiofur on account of its sensitivity, rapidity and cost-effectiveness. In addition, it could have significant application for the determination of ceftiofur in other food products.

中文翻译：

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柱前衍生HPLC-UV法快速，灵敏地测定蜂蜜和兽药中的头孢噻呋

一种新型，灵敏，快速的高效液相色谱（HPLC）方法，通过1,2-萘醌-4-磺酸酯的预柱衍生化法测定头孢噻呋。在5分钟内基于核壳技术在Kinetex C18色谱柱上进行了分析。在254 nm的UV检测下，以1.0 mL / min的流速等度泵入由乙腈-水（50:50，v / v）组成的流动相。仔细评估和优化了影响衍生化反应和分离条件的因素。该方法在浓度范围为45–450 ng / mL范围内是线性的，检出限为3.29 ng / mL，定量限为10.97 ng / mL。该新方法已成功用于兽药和蜂蜜中头孢噻呋的分析，平均回收率分别为100.78％和98. 83％。由于本方法灵敏，快速且具有成本效益，因此适合并有利于头孢噻呋的分析。此外，它在其他食品中头孢噻呋的测定中也可能具有重要的应用。