This study examined the occurrence and molecular basis for antibiotic-resistant staphylococci from the wastewater treatment plant and grey-water samples in Obafemi Awolowo University, Nigeria. Standard microbiological techniques and molecular methods were utilized. The species identified (MALDI score >1.7) comprised S. saprophyticus (19), S. cohnii (8), S. sciuri (7), S. aureus (4), S. epidermidis (3), S. warneri (2), S. equorum (1), S. haemolyticus (1), S. nepalensis (1), S. condimenti (1), and S. pasteuri (1). Resistance to trimethoprim, tetracycline and cefoxitin were observed in 78.3% (47/60), 36.7% (22/60) and 25% (15/60) of the isolates, respectively. The rate of multidrug resistance was 53.3% (32/60) and observed in eight species from different sampling sites. Seven (S. sciuri; n = 5; S. aureus; n = 1; S. warneri; n = 1) of the 20 selected (representing the various staphylococcal species and antibiotypes) isolates were mecA-positive. Furthermore, the tetK gene was detected in nine isolates, six with dfrA, and four were positive for the dfrG gene. One S. aureus was mecA, tetK and dfrG gene positive. The study provides insights on antibiotic-resistant staphylococci from a non-clinical setting and highlights the need for active surveillance to understand the burden of antimicrobial resistance in Nigeria. This is key to improve synergy across the human, animal and environmental health sectors in Nigeria.

这项研究检查了尼日利亚Obafemi Awolowo大学污水处理厂和灰水样品中抗生素耐药葡萄球菌的发生及其分子基础。使用标准的微生物学技术和分子方法。物种鉴定（MALDI评分> 1.7）由腐生葡萄球菌（19），科氏葡萄球菌（8），松鼠葡萄球菌（7），金黄色葡萄球菌（4），表皮葡萄球菌（3），S。沃（2 ），S. equorum（1），溶血葡萄球菌（1），S。冬瓜（1），S。condimenti（1），和S. pasteuri（1）。在分离株中分别观察到对甲氧苄啶，四环素和头孢西丁有抗药性，分别为78.3％（47/60），36.7％（22/60）和25％（15/60）。多药耐药率是53.3％（32/60），在不同采样点的八种物种中观察到。所选的20种分离株（代表各种葡萄球菌和抗生物型）中有7种（S. sciuri；n = 5；金黄色葡萄球菌；n = 1；S。warneri；n = 1）是mecA阳性的。此外，在9个分离株中检测到tetK基因，其中6个具有dfrA，其中4个为dfrG基因阳性。一金黄色葡萄球菌是mecA，tetK和dfrG基因阳性。这项研究提供了非临床背景下对抗生素耐药的葡萄球菌的见解，并强调需要积极监测以了解尼日利亚抗菌药物耐药性的负担。这是提高尼日利亚人类，动物和环境卫生部门协同作用的关键。