Chlorine-resistant bacteria threaten drinking water safety in water distribution systems. In this study, a novel chlorine-resistant bacterium identified as Gordonia was isolated from the drinking water supply system of Jinan City for the first time. We examined the resistance and inactivation of the isolate by investigating cell survival, changes in cell morphology, and the permeability of cell membranes exposed to chlorine. After 240 min chlorine exposure, the chlorine residual was greater than 0.5 mg L⁻¹ and the final inactivation was about 3 log reduction, which showed that the Gordonia strain had high chlorine tolerance. Flow-cytometric analysis indicated that, following sodium hypochlorite treatments with increasing membrane permeability, culturable cells enter a viable but nonculturable state and then die. We also investigated the inactivation kinetics of Gordonia following chlorine dioxide and ultraviolet radiation treatment. We found that these treatments can effectively inactivate Gordonia, which suggests that they may be used for the regulation of chlorine-resistant microorganisms.

耐氯细菌威胁着供水系统中饮用水的安全。在这项研究中，首次从济南市的饮用水供应系统中分离出了一种新型的耐氯细菌，名为高登菌。我们通过研究细胞存活率，细胞形态变化和暴露于氯的细胞膜通透性，检查了分离物的抗性和灭活性。氯接触240分钟后，氯残留量大于0.5 mg L ^-1，最终灭活约为3 log减少，这表明Gordonia该菌株具有较高的耐氯性。流式细胞仪分析表明，在次氯酸钠处理后，膜通透性增加，可培养细胞进入存活但不可培养的状态，然后死亡。我们还研究了二氧化氯和紫外线辐射处理后的Gordonia失活动力学。我们发现这些处理可以有效地灭活Gordonia，这表明它们可以用于调节耐氯微生物。