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Molecular detection of beta-lactamase blaCTX-M group 1 in Escherichia coli isolated from drinking water in Khartoum State
Journal of Water & Health ( IF 2.5 ) Pub Date : 2020-12-01 , DOI: 10.2166/wh.2020.160
Hisham N. Altayb 1 , Eman Khalid Salih 2 , Ehssan H. Moglad 3
Affiliation  

This study aimed to detect the blaCTX-M group 1 in Escherichia coli (E. coli) isolated from drinking water in Khartoum State. Two hundred and eighty water samples were collected randomly from different areas, places, and sources from the state and examined for the presence of E. coli as a fecal contamination indicator. Isolation and identification of E. coli were performed using culture characteristics on different culture media and biochemical reactions. An antimicrobial sensitivity test was performed for all isolated E. coli using agar disk diffusion method. DNA was extracted by boiling method, and bacterial genomic DNA used as a template to detect blaCTX-M group 1 by PCR. Results showed 86 (30.7%) E. coli were isolated out of 280 water samples. Antimicrobial susceptibility testing revealed the highest resistant percentage was 59% for tetracycline, followed by 35% for gentamycin, while for chloramphenicol and cefotaxime was 22 and 20%, respectively. blaCTX-M group 1 was detected in about 40% of all isolates. This study concludes that drinking water in Khartoum State may be contaminated with feces and might be a possible source for transferring resistant bacteria. Thus, it may be one of the critical causes of increasing reports of antimicrobial resistance in Khartoum State.



中文翻译:

喀土穆州饮用水中分离的大肠杆菌中β-内酰胺酶blaCTX-M第1组的分子检测

本研究旨在检测BLA CTX-M在第1组的大肠杆菌大肠杆菌从喀土穆州饮用水隔离)。从州的不同区域,地点和水源随机收集了280个水样本,并检查了是否存在作为粪便污染指标的大肠杆菌。利用在不同培养基上的培养特性和生化反应进行大肠杆菌的分离和鉴定。使用琼脂圆盘扩散法对所有分离的大肠杆菌进行了抗菌敏感性测试。通过煮沸法提取DNA,并以细菌基因组DNA为模板检测bla。通过PCR检测CTX-M组1。结果显示,从280个水样中分离出86个(30.7%)大肠杆菌。抗菌药敏测试显示,四环素的最高耐药率是59%,庆大霉素的耐药率是35%,而氯霉素和头孢噻肟的耐药率分别是22%和20%。在所有分离株中约有40%检测到bla CTX-M组1。这项研究得出的结论是,喀土穆州的饮用水可能被粪便污染,并且可能是转移耐药菌的可能来源。因此,这可能是喀土穆州增加抗菌素耐药性报道的重要原因之一。

更新日期:2020-12-17
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