Recent investigations of East Asian freshwater mussel genera Nodularia, Middendorffinaia and Inversiunio confirmed validity of the taxa, while their composition and taxonomic uncertainty concerning classification of these genera within a specific subfamily are still in question. The aim of the present study is to clarify the taxonomy and systematics of Nodularia and Middendorffinaia genera from the Russian Far East and Inversiunio from Japan, using a multiple dataset approach that combines morphology of glochidia, detailed conchological analyses with morphometry of adult and glochidial shells, and a molecular technique involving COI barcoding marker. As a result, three genetically distinct and highly divergent lineages were recognized confirming the existence of three distinct genera. The existence of five Nodularia species in Southeast Asia is shown. One of the sequences is shown to be incorrectly assigned to N. jourdyi, while in fact it also represents N. douglasiae, according to the BIN system. Existence of a single Nodularia species, namely N. douglasiae, and a single Middendorffinaia species, namely M. mongolica, in the Russian Far East is supported. We conclude that so-called comparatory species Nodularia amurensis, N. schrencki, and N. vladivostokensis are junior synonyms of the species Nodularia douglasiae; the comparatory species Middendorffinaia ussuriensis and M. dulkeitiana are junior synonyms of the species Middendorffinaia mongolica. Within Inversiunio specimens from Japan four species were recognized based on types of glochidial microsculpture of shells and genetic data. Gene sequences of Inversiunio verrucosus, endemic to the Seomjingang River basin in South Korea, and I. yanagawensis from Kyushu Island in Japan are very similar and they can be regarded as the same species, with Inversiunio verrucosus as a junior synonym of Inversiunio yanagawensis. Mussels from Honshu Island first referred to I. yanagawensis are shown to be a separate species.

东亚蚌属的最近的研究节球，Middendorffinaia和Inversiunio证实类群的有效性，而其组成和分类学的不确定性有关的特定范围内，这些属的分类亚科仍然有问题。本研究的目的是使用多数据集方法（结合成球孢子虫的形态，详细的贝壳菌学分析以及成虫和毛状壳的形态），阐明俄罗斯远东和日本的Inversiunio的结节虫属和Middendorffinaia属的分类学和系统学，和涉及COI的分子技术条码标记。结果，确认了三个遗传上不同且高度不同的谱系，证实了三个不同属的存在。显示了东南亚存在五个结节菌物种。一个序列的被示出为被错误地分配到N. jourdyi，而实际上它也代表N. douglasiae，根据BIN系统。支持在俄罗斯远东地区存在一个单一的结节菌物种，即道格拉斯猪笼草（N. douglasiae）和一个单一的Middendorffinaia物种，即M. mongolica。我们得出的结论是，所谓的比较种阿穆尔氏结节菌（N. schrencki）和N. vladivostokensis是Nodularia douglasiae种的初级同义词; 比较树种Midsdorfdorffinaia ussuriensis和M. dulkeitiana是蒙古种Middendorffinaia mongolica的初级同义词。根据贝壳的球状显微雕刻的类型和遗传数据，在日本的Inversiunio标本中识别出4种。Vereucosuni verrucosus的基因序列，在韩国的Seomjingang流域特有，与日本九州岛的yanagawensis相似，可以被认为是与Inversiunio verrucosus相同的物种。作为延蚊的初级同义词。来自本州岛的贻贝最初被称为yanagawensis，被证明是一个单独的物种。