Abstract

The alkaline cleavage of two types of sugar nucleotides has been studied by ¹H and ³¹P NMR in order to obtain information on the stability and decomposition pathways in aqueous solutions under alkaline conditions. The reaction of glucose 1-UDP is straightforward, and products are easy to identify. The results obtained with ribose 5-UDP and ribose 5-phosphate reveal, in contrast, a more complex reaction system than expected, and the identification of individual intermediate species was not possible. Even though definite proof for the mechanisms previously proposed could not be obtained, all the spectroscopic evidence is consistent with them. Results also emphasise the significant effect of conditions, pH, ionic strength, and temperature, on the reactivity under chemical conditions.

摘要

为了获得有关碱性条件下水溶液中稳定性和分解途径的信息，已通过¹ H和³¹ P NMR研究了两种类型的糖核苷酸的碱性裂解。葡萄糖1-UDP的反应简单明了，产品易于识别。相比之下，使用核糖5-UDP和核糖5-磷酸所获得的结果表明，反应系统比预期的更为复杂，并且无法鉴定单个中间物种。即使无法获得先前提出的机制的确切证据，所有光谱学证据都与它们一致。结果还强调了条件，pH，离子强度和温度对化学条件下反应性的重大影响。