Abstract

Artemisia roxburghiana is used for the management of diabetes mellitus in the Indian subcontinent. The present work aimed to validate the traditional claim of the plant in diabetes mellitus. In vitro studies were conducted using α-glucosidase and α-amylase assays whereas streptozotocin-nicotinamide-induced diabetic Wistar rats were used for in vivo study. The aqueous-ethanol extract from the aerial parts was found to exhibit α-glucosidase and α-amylase inhibitory activities with the IC₅₀ values of 31.0 and 17.2 mg/mL, respectively when compared with acarbose (IC₅₀ = 8.6 and 16.25 mg/mL, respectively). The extract showed a significant glucose-lowering effect in diabetic rats at the doses of 200 and 400 mg/kg in a dose-dependent manner, while acarbose (10 mg/kg) was used as a standard. The results revealed that A. roxburghiana aerial parts showed antidiabetic activity via inhibiting α-glucosidase and α-amylase enzymes. The present study also validated the ethnomedicinal claim of the plant in diabetes mellitus.

摘要

蒿（Artemisia roxburghiana）用于印度次大陆的糖尿病管理。目前的工作旨在验证糖尿病患者对植物的传统主张。使用α-葡萄糖苷酶和α-淀粉酶测定法进行了体外研究，而链脲佐菌素-烟酰胺诱导的糖尿病Wistar大鼠用于体内研究。从地上部分水-乙醇提取物被发现表现出α葡糖苷酶和α淀粉酶抑制活性用IC ₅₀的31.0和17.2的值毫克/毫升，分别与阿卡波糖相比（IC ₅₀分别为8.6和16.25 mg / mL）。在200和400 mg / kg的剂量下，该提取物在糖尿病大鼠中显示出显着的降糖作用，且剂量依赖性，而阿卡波糖（10 mg / kg）被用作标准。结果表明，A。roxburghiana地上部分通过抑制α-葡萄糖苷酶和α-淀粉酶具有抗糖尿病作用。本研究还证实了该植物在糖尿病中的人种学主张。