As part of a project to document the diversity of larval invertebrates on both coasts of Panama, we collected and photographed 141 larval asteroids and sequenced fragments of their mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal DNA. We uncovered 10 Caribbean operational taxonomic units (OTUs) and five Pacific OTUs. We could identify six of the 15 OTUs based on >99% similarity with reference sequences in GenBank: The Pacific species Astropecten verrilli and Pentaceraster cumingi and the Caribbean species Astropecten marginatus, Astropecten antillensis, Oreaster reticulatus, and Mithrodia clavigera. Two other OTUs were placed in BINs in the Barcode of Life Database (BOLD) with unpublished sequences that were identified as Pharia pyramidata from the Pacific and Valvaster striatus, now known from the Caribbean as well as the Indo‐West Pacific. The remaining seven species appear likely to belong to Luidia, as 16S sequences from each have 87%–95% identity with various species of Luidia, and the sequences nest among species of Luidia in neighbor‐joining trees. The low diversity of asteroid larvae reflects ~10% of the diversity of adult sea stars reported from Panama, and highlights the need for broader collection efforts and improved coverage of DNA barcode reference sequences for Luidia and other soft‐bottom species.

中文翻译：

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我想知道你是什么：DNA条形码可以识别巴拿马的幼虫小行星吗？

作为记录巴拿马两个海岸上幼虫无脊椎动物多样性的项目的一部分，我们收集并拍摄了141个幼虫小行星，并对它们的线粒体细胞色素C氧化酶亚基I（COI）和16S核糖体DNA进行了测序。我们发现了10个加勒比海业务分类单位（OTU）和五个太平洋OTU。基于与GenBank中参考序列的> 99％相似性，我们可以鉴定出15个OTU中的六个：太平洋物种Astropecten verrilli和Pentaceraster cumingi以及加勒比海物种Astropecten marginatus，Astropecten antillensis，Oreaster reticulatus和Mithrodia clavigera。其他两个OTU被放置在生命条形码数据库（BOLD）中的BIN中，其未公开序列被鉴定为来自Pacific和Valvaster striatus的Pharia pyramidata，现在从加勒比海地区和印度西太平洋地区都知道。其余的7个物种似乎可能属于Luidia，因为每个物种的16S序列与Luidia的各种物种具有87％–95％的同一性，并且这些序列嵌套在Luidia的物种之间在相邻的树木中。小行星幼虫的低多样性反映了巴拿马报道的约10％的成年海星多样性，并突出表明需要为Luidia和其他软底物种进行更广泛的收集工作并提高DNA条码参考序列的覆盖范围。