Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells,FEBS Open Bio

当前位置： X-MOL 学术 › FEBS Open Bio › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-12-17 , DOI: 10.1002/2211-5463.13066
Chihiro Miya _{1,

2} , Marni E Cueno ₂ , Ryuta Suzuki _{1,

2} , Shuichiro Maruoka ₃ , Yasuhiro Gon ₃ , Tadayoshi Kaneko ₁ , Yoshiyuki Yonehara ₁ , Kenichi Imai ₂

Affiliation

Porphyromonas gingivalis (Pg) is a periodontopathic pathogen that may affect MUC5AC‐related mucus hypersecretion along airway epithelial cells. Here, we attempted to establish whether Pg virulence factors (lipopolysaccharide, FimA fimbriae, gingipains) affect MUC5AC in immortalized and primary bronchial cells. We report that MUC5AC gene expression and protein levels are affected by Pg culture supernatant, but not by lipopolysaccharide or FimA fimbriae. Cells treated with either Pg single (Kgp or Rgp) or double (Kgp/Rgp) mutants had altered levels of MUC5AC gene expression and protein levels, and MUC5AC staining of double mutant‐treated mouse lung cells showed that MUC5AC protein levels were unaffected. Taken together, we propose that Pg gingipains may be the primary virulence factor that influences both MUC5AC gene expression and protein levels.

中文翻译：

Porphyromonas gingivalis gingipains 可能影响呼吸道上皮细胞中 MUC5AC 基因的表达和蛋白水平

牙龈卟啉单胞菌(Pg) 是一种牙周病病原体，可能会影响沿气道上皮细胞的 MUC5AC 相关粘液过度分泌。在这里，我们试图确定 Pg 毒力因子（脂多糖、FimA 菌毛、牙龈蛋白酶）是否影响永生化和初级支气管细胞中的 MUC5AC。我们报告 MUC5AC 基因表达和蛋白质水平受 Pg 培养上清液的影响，但不受脂多糖或 FimA 菌毛的影响。用 Pg 单（Kgp 或 Rgp）或双（Kgp/Rgp）突变体处理的细胞的 MUC5AC 基因表达水平和蛋白质水平发生了变化，双突变体处理的小鼠肺细胞的 MUC5AC 染色显示 MUC5AC 蛋白水平不受影响。总之，我们提出 Pg gingipains 可能是影响 MUC5AC 基因表达和蛋白质水平的主要毒力因子。

更新日期：2021-02-11

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11