The calcium‐dependent protein kinase ZmCDPK7 functions in heat‐stress tolerance in maize,Journal of Integrative Plant Biology

当前位置： X-MOL 学术 › J. Integr. Plant Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

The calcium‐dependent protein kinase ZmCDPK7 functions in heat‐stress tolerance in maize
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-12-17 , DOI: 10.1111/jipb.13056
Yulong Zhao ₁ , Hanwei Du ₁ , Yankai Wang ₁ , Huali Wang ₁ , Shaoyu Yang ₁ , Chaohai Li ₁ , Ning Chen ₁ , Hao Yang ₁ , Yihao Zhang ₁ , Yulin Zhu ₁ , Luyao Yang ₁ , Xiuli Hu ₁

Affiliation

Global warming poses a serious threat to crops. Calcium‐dependent protein kinases (CDPKs)/CPKs play vital roles in plant stress responses, but their exact roles in plant thermotolerance remains elusive. Here, we explored the roles of heat‐induced ZmCDPK7 in thermotolerance in maize. ZmCDPK7‐overexpressing maize plants displayed higher thermotolerance, photosynthetic rates, and antioxidant enzyme activity but lower H₂O₂ and malondialdehyde (MDA) contents than wild‐type plants under heat stress. ZmCDPK7‐knockdown plants displayed the opposite patterns. ZmCDPK7 is attached to the plasma membrane but can translocate to the cytosol under heat stress. ZmCDPK7 interacts with the small heat shock protein sHSP17.4, phosphorylates sHSP17.4 at Ser‐44 and the respiratory burst oxidase homolog RBOHB at Ser‐99, and upregulates their expression. Site‐directed mutagenesis of sHSP17.4 to generate a Ser‐44‐Ala substitution reduced ZmCDPK7's enhancement of catalase activity but enhanced ZmCDPK7's suppression of MDA accumulation in heat‐stressed maize protoplasts. sHSP17.4, ZmCDPK7, and RBOHB were less strongly upregulated in response to heat stress in the abscisic acid‐deficient mutant vp5 versus the wild type. Pretreatment with an RBOH inhibitor suppressed sHSP17.4 and ZmCDPK7 expression. Therefore, abscisic acid‐induced ZmCDPK7 functions both upstream and downstream of RBOH and participates in thermotolerance in maize by mediating the phosphorylation of sHSP17.4, which might be essential for its chaperone function.

中文翻译：

钙依赖性蛋白激酶ZmCDPK7在玉米耐热胁迫中的作用

全球变暖对农作物构成了严重威胁。钙依赖性蛋白激酶（CDPKs）/ CPK在植物胁迫响应中起着至关重要的作用，但它们在植物耐热性中的确切作用仍然难以捉摸。在这里，我们探讨了热诱导的ZmCDPK7在玉米耐热性中的作用。ZmCDPK7过表达的玉米植物在热胁迫下显示出比野生型植物更高的耐热性，光合速率和抗氧化酶活性，但具有较低的H ₂ O ₂和丙二醛（MDA）含量。ZmCDPK7击倒的植物显示出相反的模式。ZmCDPK7附着在质膜上，但在热应激下可易位到细胞质中。ZmCDPK7与小的热激蛋白sHSP17.4相互作用，在Ser-44磷酸化sHSP17.4，在Ser-99磷酸化呼吸猝发氧化酶同系物RBOHB，并上调其表达。sHSP17.4的定点诱变产生Ser-44-Ala取代减少了ZmCDPK7对过氧化氢酶活性的增强，但增强了ZmCDPK7对热胁迫的玉米原生质体中MDA积累的抑制。与野生型相比，脱落酸缺陷型突变体vp 5中的sHSP17.4，ZmCDPK7和RBOHB响应热应激的上调程度较低。抑制RBOH抑制剂的预处理sHSP17.4和ZmCDPK7表达。因此，脱落酸诱导的ZmCDPK7在RBOH的上游和下游均起作用，并通过介导sHSP17.4的磷酸化参与玉米的耐热性，这可能是其伴侣功能所必需的。

更新日期：2020-12-17

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11