Introduction of glycine synthase enables uptake of exogenous formate and strongly impacts the metabolism in Clostridium pasteurianum,Biotechnology and Bioengineering

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Introduction of glycine synthase enables uptake of exogenous formate and strongly impacts the metabolism in Clostridium pasteurianum
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-12-17 , DOI: 10.1002/bit.27658
Yaeseong Hong ₁ , Philipp Arbter ₁ , Wei Wang ₁ , Lilian N Rojas ₁ , An-Ping Zeng _{1,

2}

Affiliation

Autotrophic or mixotrophic use of one‐carbon (C1) compounds is gaining importance for sustainable bioproduction. In an effort to integrate the reductive glycine pathway (rGP) as a highly promising pathway for the assimilation of CO₂ and formate, genes coding for glycine synthase system from Gottschalkia acidurici were successfully introduced into Clostridium pasteurianum, a non‐model host microorganism with industrial interests. The mutant harboring glycine synthase exhibited assimilation of exogenous formate and reduced CO₂ formation. Further metabolic data clearly showed large impacts of expression of glycine synthase on the product metabolism of C. pasteurianum. In particular, 2‐oxobutyrate (2‐OB) was observed for the first time as a metabolic intermediate of C. pasteurianum and its secretion was solely triggered by the expression of glycine synthase. The perturbation of C1 metabolism is discussed regarding its interactions with pathways of the central metabolism, acidogenesis, solventogenesis, and amino acid metabolism. The secretion of 2‐OB is considered as a consequence of metabolic and redox instabilities due to the activity of glycine synthase and may represent a common metabolic response of Clostridia in enhanced use of C1 compounds.

中文翻译：

甘氨酸合酶的引入能够吸收外源甲酸并强烈影响巴氏梭菌的代谢

自养或混养使用单碳 (C1) 化合物对于可持续生物生产越来越重要。为了整合还原甘氨酸途径 (rGP) 作为同化 CO _{2和甲酸的非常有前景的途径，将来自}Gottschalkia acidurici的甘氨酸合酶系统编码基因成功引入巴氏梭菌，这是一种具有工业生产能力的非模式宿主微生物。利益。含有甘氨酸合酶的突变体表现出外源甲酸的同化和减少的CO ₂形成。进一步的代谢数据清楚地表明甘氨酸合酶的表达对巴氏梭菌的产物代谢有很大影响. 特别是，首次观察到 2-氧代丁酸 (2-OB) 作为巴氏梭菌的代谢中间体，其分泌仅由甘氨酸合酶的表达触发。C1 代谢的扰动讨论了它与中枢代谢、酸生成、溶剂生成和氨基酸代谢途径的相互作用。2-OB 的分泌被认为是由于甘氨酸合酶的活性导致的代谢和氧化还原不稳定性的结果，并且可能代表梭状芽胞杆菌在增强 C1 化合物使用方面的常见代谢反应。

更新日期：2021-02-18

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