The mosquito Culex pipiens is the most widely distributed dipteran species in all regions of Egypt and the principal vector of Wuchereria bancrofti and certain arboviruses in human beings. For controlling C. pipiens vector, biological tools (e.g., larvivorous fish and bioinsecticides) are more potent and safer options to the environment, human beings, and beneficial organisms than chemical pesticides. The efficiency of O. niloticus juveniles as predatory fish species and two bioinsecticides, spinosad 24% and spinetoram 12%, was investigated against the C. pipiens developmental stages in the laboratory. The first trial evaluated the predatory efficacy of small-sized O. niloticus (2.1–2.6 cm; 250–315 mg) and large-sized O. niloticus (2.5–3.2 cm; 250–315 mg) against the 3rd larvae and pupae of C. pipiens. This is the first report in Egypt confirming the predation potential of O. niloticus as efficient predatory fish against the immature C. pipiens. Large-sized O. niloticus predated a greater number of 3rd of C. pipiens larvae and pupae than the small-sized ones. Furthermore, the daily consumption of C. pipiens larvae by small- and large-sized O. niloticus was significantly higher than the pupae. The second trial assessed the toxicity efficacy of spinosad 24% and spinetoram 12% against C. pipiens larvae and pupae. The results confirmed that the tested bioinsecticides showed higher potency toward C. pipiens larvae than pupae after exposure for 24 h and 48 h. Spinosad was more toxic toward 3rd C. pipiens larvae (LC₅₀ = 0.013 and 0.003 mg/L) and pupae (LC₅₀ = 320.69 and 44.28 mg/L) than spinetoram after 24 and 48 h. Herein, O. niloticus juveniles (as promising native predatory fish) and spinosyns bioinsecticides were more effective against C. pipiens in the larval stage than in the pupal stage. In conclusion, Nile tilapia juveniles and biorational compounds, spinosad 24% and spinetoram 12%, might be considered as promising and favorable environmental biological agents for controlling C. pipiens in Egypt. However, further trials are needed to investigate the potential of these agents in the control of this mosquito vector under field conditions.

蚊子库蚊（Pilex pipiens）是埃及所有地区分布最广泛的二倍体物种，是人类黑腹果蝇（Wuchereria bancrofti）和某些虫媒病毒的主要媒介。为了控制C.蚊载体，生物工具（例如，larvivorous鱼类和生物杀虫剂）是更有效和更安全的选择对环境，人类和有益生物不是化学杀虫剂。的效率尼罗罗非鱼幼体掠夺鱼类和两个生物杀虫剂，多杀菌素为24％和乙基多杀菌素12％时，对所研究的C.蚊在实验室发育阶段。第一次试验评估了小型罗非鱼的掠食性功效（2.1–2.6厘米； 250–315毫克）和大型罗非鱼（2.5–3.2厘米； 250–315毫克）抵制淡色梭菌的第三幼虫和p。这是埃及的第一份报告，证实了尼罗罗非鱼（O. niloticus）具有潜在的捕食能力，可以抵抗未成熟的pipiens。大型的O. niloticus比小型的C. pipiens幼虫和p要多。此外，小型和大型的尼罗罗非鱼（O. niloticus）每天对pipiens pi.ens幼虫的消费明显高于the。第二项试验评估了多杀菌素24％和多杀菌素12％对C. pipiens幼虫和p。结果证实，经测试的生物杀虫剂在暴露24 h和48 h后，对pipiens pipiens幼虫的效力比对pupae的效力高。多杀菌素是毒性更大朝向第三C.蚊幼虫（LC ₅₀  = 0.013和0.003毫克/升）和蛹（LC ₅₀  = 320.69和44.28毫克/升）24和48小时后小于乙基多杀菌素。在此，尼罗罗非鱼幼体（作为有前途的天然掠食性鱼类）和多杀菌素生物杀虫剂对pipiens的杀灭作用更强在幼虫期比在stage期。总之，尼罗罗非鱼的幼鱼和生物合理的化合物，多杀菌素为24％，多杀菌素为12％，可能被认为是控制埃及pipiens的有前途和有利的环境生物制剂。但是，需要进一步的试验来研究这些试剂在田间条件下控制这种蚊媒的潜力。