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Monomer conversion, release and cytotoxicity analyses of experimental GDMA-P based adhesives
International Journal of Adhesion and Adhesives ( IF 3.2 ) Pub Date : 2021-03-01 , DOI: 10.1016/j.ijadhadh.2020.102792
Rodolfo Sousa-Lima , Ana Justo-Fernandes , Marina Kaizer , Cristina Isolan , Rafael Moraes , Cícero Aragão , Carlos Barboza , Isauremi Assunção , Boniek Borges

Abstract This study aimed to evaluate the effects of a photoinitiator system and acidic monomer (GDMA-P) concentrations on self-etch experimental dental adhesive degree of conversion (DC), monomer release and cytotoxicity. Three monomer blends containing different GDMA-P/HEMA/UDMA ratios were produced: 1/3/3; 2/3/2; and 3/3/1. CQ + EDMAB; BAPO or CQ + BAPO + EDMAB were used as photoinitiator systems. DFI was added as co-initiator, and a water/ethanol mixture was used as solvent. DC was evaluated by Fourier transform infrared spectroscopy (FTIR) (n=10). Monomer release was identified by ultra-high performance liquid chromatography (UHPLC) (n=6). The Alamar Blue assay was used to test NIH-3T3 fibroblast cell viability (n=8). A two-way ANOVA and Tukey post-hoc test were used for the data analyses (p

中文翻译:

基于 GDMA-P 的实验性粘合剂的单体转化、释放和细胞毒性分析

摘要 本研究旨在评估光引发剂体系和酸性单体 (GDMA-P) 浓度对自蚀刻实验牙科粘附转化度 (DC)、单体释放和细胞毒性的影响。生产了三种含有不同 GDMA-P/HEMA/UDMA 比率的单体混合物:1/3/3;2/3/2; 和 3/3/1。CQ + EDMAB; BAPO 或 CQ + BAPO + EDMAB 用作光引发剂体系。添加 DFI 作为共引发剂,并使用水/乙醇混合物作为溶剂。DC 通过傅里叶变换红外光谱 (FTIR) (n=10) 进行评估。通过超高效液相色谱 (UHPLC) (n=6) 鉴定单体释放。Alamar Blue 测定用于测试 NIH-3T3 成纤维细胞的活力 (n=8)。使用双向方差分析和 Tukey 事后检验进行数据分析 (p
更新日期:2021-03-01
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