Abstract Aim Novel k21 PLGA nanoparticles (nano-PLGA-RF/VE-TPGS0.50⁞k21) with synergistic effect of VE-TPGS with riboflavin-5-phospshate solution were used to analyze structural integrity, chemical interactions, antimicrobial resistance, mechanical properties as a delivery tool to acid-demineralized dentine-substrates. Materials and Methods Synthesized spherical nano-PLGA ⋮ RF/VE-TPGS0.50⁞k21 were assessed for drug-encapsulation and release, antibacterial-profile, MTT, molecular simulation for MMP-2 and MMP-9 and Raman spectroscopy. Results k21 favoured increase in colloidal-size with positive zeta potential. Bands indicated k21 entrapment as nanoparticles were confined inside tubules with no effect on μTBS. XP-GScores were −2.383 and −4.383 for MMP-2 and -9. HYP liberation recorded with collagenase degradation resistance in nano-PLGA ⋮ RF/VE-TPGS0.50⁞k21 specimens. Conclusion Nano-PLGA ⋮ RF/VE-TPGS0.50⁞k21 specimens exhibited superior antibacterial/antibiofilm effects against cariogenic biofilms after bonding-resins infiltration without adversely affecting bond strength.

中文翻译：

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载有季铵硅烷和核黄素的 PLGA 纳米颗粒在粘合剂牙科中的潜在应用

摘要 目的 新型 k21 PLGA 纳米粒子 (nano-PLGA-RF/VE-TPGS0.50⁞k21) 具有 VE-TPGS 与核黄素-5-磷酸溶液的协同作用，用于分析结构完整性、化学相互作用、抗微生物性、机械性能作为酸脱矿牙本质基质的传递工具。材料和方法 对合成的球形纳米 PLGA ⋮ RF/VE-TPGS0.50⁞k21 进行药物包封和释放、抗菌谱、MTT、MMP-2 和 MMP-9 的分子模拟以及拉曼光谱评估。结果 k21 有利于增加胶体大小并具有正 zeta 电位。条带表明 k21 被截留，因为纳米颗粒被限制在小管内，对 μTBS 没有影响。MMP-2 和 -9 的 XP-GScores 为 -2.383 和 -4.383。用纳米 PLGA ⋮ RF/VE-TPGS0.50⁞k21 样品中的胶原酶降解抗性记录 HYP 释放。结论 Nano-PLGA ⋮ RF/VE-TPGS0.50⁞k21 样品在粘合树脂浸润后对致龋生物膜表现出优异的抗菌/抗生物膜作用，而不会对粘合强度产生不利影响。