The deformation of the mouse astrocytic lamina (AL) and adjacent peripapillary sclera (PPS) was measured in response to elevated intraocular pressure. We subjected explanted mouse eyes to inflation testing, comparing control eyes to those 3 days and 6 weeks after induction of ocular hypertension (OHT) via ocular microbead injection. Laser scanning microscopy was used with second harmonic generation to image the collagenous PPS and two-photon fluorescence to image transgenic fluorescent astrocytes in the AL. Digital volume correlation was applied to calculate strains in the PPS and AL. The specimen-averaged strains were biaxial in the AL and PPS, with greater strain overall in the x- than y-direction in the AL and greater strain in the θ- than the r-direction in the PPS. Strains increased after 3-day OHT, with greater strain overall in the 3-day AL than control AL, and greater circumferential strain in the 3-day PPS than control PPS. In the 6-week OHT eyes, AL and PPS strains were similar overall to controls. This experimental glaucoma model demonstrated a dynamic change in the mechanical behaviour of the AL and PPS over time at the site of neuronal injury and remodelling in glaucoma.

中文翻译：

---

青光眼小鼠模型视神经乳头和视乳头周围巩膜的生物力学


测量小鼠星形细胞层（AL）和邻近视盘周围巩膜（PPS）对眼内压升高的反应的变形。我们对移植的小鼠眼睛进行充气测试，将对照眼睛与通过眼部微珠注射诱导高眼压 (OHT) 后 3 天和 6 周的眼睛进行比较。使用激光扫描显微镜和二次谐波生成对胶原 PPS 进行成像，并使用双光子荧光对 AL 中的转基因荧光星形胶质细胞进行成像。应用数字体积相关性来计算 PPS 和 AL 中的应变。 AL 和 PPS 中的样本平均应变是双轴的，AL 中 x 方向的整体应变大于 y 方向的应变，PPS 中 θ 方向的应变大于 r 方向的应变。 3 天 OHT 后应变增加，3 天 AL 中的整体应变比对照 AL 更大，3 天 PPS 中的圆周应变比对照 PPS 更大。在 6 周 OHT 眼中，AL 和 PPS 菌株总体与对照组相似。该实验性青光眼模型证明了青光眼神经元损伤和重塑部位的 AL 和 PPS 机械行为随时间的动态变化。