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Electrochemical H2O2 biosensor based on horseradish peroxidase encapsulated protein nanoparticles with reduced graphene oxide-modified gold electrode
Nano Convergence ( IF 13.4 ) Pub Date : 2020-12-16 , DOI: 10.1186/s40580-020-00249-0
Jeong-Hyeop Shin , Myeong-Jun Lee , Jin-Ha Choi , Ji-ae Song , Tae-Hwan Kim , Byung-Keun Oh

In this study, an electrochemical biosensor composed of a horseradish peroxidase (HRP)-encapsulated protein nanoparticles (HEPNP) was fabricated for the sensitive and selective detection of H2O2. The HEPNP has a three-dimensional structure that can contain a large amount of HRP; therefore, HEPNP can amplify the electrochemical signals necessary for the detection of H2O2. Furthermore, reduced graphene oxide (rGO) was used to increase the efficiency of electron transfer from the HEPNP to an electrode, which could enhance the electrochemical signal. This biosensor showed a sensitive electrochemical performance for detection of H2O2 with signals in the range from 0.01–100 μM, and it could detect low concentrations up to 0.01 μM. Furthermore, this biosensor was operated against interferences from glucose, ascorbic acid, and uric acid. In addition, this fabricated H2O2 biosensor showed selective detection performance in human blood serum. Therefore, the proposed biosensor could promote the sensitive and selective detection of H2O2 in clinical applications.

中文翻译:

基于辣根过氧化物酶包裹的蛋白质纳米粒子和氧化石墨烯修饰的金电极的电化学H 2 O 2传感器

在这项研究中,由辣根过氧化物酶(HRP)封装的蛋白质纳米颗粒(HEPNP)组成的电化学生物传感器被制造用于H2O2的灵敏和选择性检测。HEPNP具有三维结构,可以包含大量的HRP。因此,HEPNP可以放大检测H2O2所需的电化学信号。此外,还原的氧化石墨烯(rGO)用于提高电子从HEPNP到电极的转移效率,这可以增强电化学信号。这种生物传感器对H2O2的检测表现出灵敏的电化学性能,其信号范围为0.01–100μM,它可以检测低至0.01μM的低浓度。此外,该生物传感器可抵抗葡萄糖,抗坏血酸和尿酸的干扰。此外,这种制造的H2O2生物传感器在人血清中表现出选择性检测性能。因此,提出的生物传感器可以促进临床应用中H2O2的灵敏和选择性检测。
更新日期:2020-12-16
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