Hematopoietic stem cells (HSCs) are a rare population of cells that reside mainly in the bone marrow and are capable of generating and fulfilling the entire hematopoietic system upon differentiation. Thirty-six healthy donors, attending the HSCT center to donate their bone marrow, were categorized according to their age into child (0-12 years), adolescence (13-18 years), and adult (19-59 years) groups, and gender into male and female groups. Then, the absolute number of HSCs and mature immune cells in their harvested bone marrow was investigated. Here, we report that the absolute cell number can vary considerably based on the age of the healthy donor, and the number of both HSCs and immune cells declines with advancing age. The gender of the donor (male or female) did not have any impact on the number of the HSCs and immune cells in the bone marrow. In conclusion, since the number of HSCs plays a pivotal role in the clinical outcome of allogeneic HSC transplantations, identifying a younger donor regardless the gender is critical.

中文翻译：

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年龄和性别对造血干细胞和骨髓微环境免疫环境的影响

造血干细胞 (HSC) 是一种罕见的细胞群，主要存在于骨髓中，能够在分化时产生和完成整个造血系统。36 名健康捐献者到 HSCT 中心捐献骨髓，按年龄分为儿童（0-12 岁）、青少年（13-18 岁）和成人（19-59 岁）组，以及性别分为男性组和女性组。然后，研究了他们收获的骨髓中 HSC 和成熟免疫细胞的绝对数量。在这里，我们报告绝对细胞数量会根据健康供体的年龄而有很大差异，并且 HSC 和免疫细胞的数量随着年龄的增长而下降。捐献者的性别（男性或女性）对骨髓中造血干细胞和免疫细胞的数量没有任何影响。总之，由于 HSC 的数量在同种异体 HSC 移植的临床结果中起着举足轻重的作用，因此无论性别如何确定年轻的供体都至关重要。