Abstract

The ultrasound-assisted aqueous two-phase extraction (UA-ATPE) was first employed to develop an effective technique for simultaneous extraction and preliminary purification of synephrine, naringin, and neohesperidin from Citrus aurantium L. fruitlets. Five types of ethanol/salts of aqueous two-phase system (ATPS) were investigated and then the extraction conditions were further optimized using single-factor experiments and response surface methodology (RSM) via Box–Behnken Design (BBD). The optimum process parameters were concluded as follows: 20.60% (w/w) K₂CO₃, 27% (w/w) ethanol, solvent-to-material ratio of 45.17:1 (g:g), 120-mesh particle size of fruitlets powder, extraction temperature of 50 °C, extraction time of 30 min, and ultrasonic power of 80 W. Under these conditions, the extraction yields of synephrine, naringin, and neohesperidin were up to 11.17 mg/g, 7.39 mg/g, and 89.27 mg/g, respectively. The yield of neohesperidin extracted by the optimal UA-ATPE was over eight times higher than that extracted by the ultrasound-assisted extraction (UAE) using conventional solvents, and the total yield of target compounds was over twice higher while the impurity content in the extract was much lower. Therefore, UA-ATPE appeared to be a highly effective and promising approach for the extraction of synephrine, naringin, and neohesperidin from C. aurantium fruitlets.

摘要

超声辅助水性两相萃取（UA-ATPE）首先用来开发用于从同时提取和脱氧肾上腺素，柚皮苷的初步纯化，并新橙皮苷的有效方法枳壳L.幼果。研究了水两相系统（ATPS）的五种乙醇/盐类型，然后通过Box-Behnken Design（BBD）使用单因素实验和响应面方法（RSM）进一步优化了提取条件。最佳工艺参数总结如下：20.60％（w / w）K ₂ CO ₃，27％（w / w）乙醇，溶剂与物料的比例为45.17：1（g：g），小颗粒粉末的120目粒径，提取温度为50°C，提取时间为30分钟以及超声在这些条件下，辛弗林，柚皮苷和新橙皮苷的提取产率分别高达11.17 mg / g，7.39 mg / g和89.27 mg / g。最佳UA-ATPE提取的新橙皮苷的收率比使用常规溶剂的超声辅助提取（UAE）提取的高八倍，目标化合物的总收率高出两倍以上，而提取物中的杂质含量更高低得多。因此，UA-ATPE似乎是从对羟福林，柚皮苷的提取的高度有效和有前景的方法，和新橙皮苷C.枳壳幼果。