Altered gene expression profile of Wolbachia pipientis wAlbB strain following transinfection from its native host Aedes albopictus to Aedes aegypti cells,Molecular Microbiology

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Altered gene expression profile of Wolbachia pipientis wAlbB strain following transinfection from its native host Aedes albopictus to Aedes aegypti cells
Molecular Microbiology ( IF 2.6 ) Pub Date : 2020-12-16 , DOI: 10.1111/mmi.14668
Cameron Bishop ₁ , Sassan Asgari ₁

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Wolbachia is an obligate intracellular bacterial symbiont prevalent among arthropods and nematodes. To survive and reproduce, Wolbachia interacts with and modifies host subcellular structures, while sensing and responding to changes within the cellular environment. In mutualistic associations, Wolbachia may provision the host with metabolites, or help to maintain the chemical homeostasis of the host cell. Some strains can rapidly invade insect populations by manipulating host reproductive biology, while also preventing viral replication, allowing their use in vector control of arthropod-borne viruses. The Aedes albopictus-derived strain wAlbB is promising in this regard. When transinfected into the Yellow fever mosquito, Aedes aegypti, wAlbB reaches high frequencies within wild populations, and strongly inhibits viral transmission. Despite its obvious potential, much is still unknown about the molecular interactions between Wolbachia and host that enable its use in vector control. Furthermore, most Wolbachia transinfection research to date has focused on host effects. In the current study, we used a cell line model to explore the effect of transinfection of wAlbB from Ae. albopictus to Ae. aegypti. Using RNA sequencing, we show that several genes associated with host-symbiont interactions were downregulated by transinfection, with the greatest downregulation exhibited by prophage-associated genes.

中文翻译：

从天然宿主白纹伊蚊转染到埃及伊蚊细胞后，Wolbachia pipientis wAlbB 菌株的基因表达谱发生改变

Wolbachia是一种在节肢动物和线虫中普遍存在的专性细胞内细菌共生体。为了生存和繁殖，沃尔巴克氏体与宿主亚细胞结构相互作用并改变宿主，同时感知和响应细胞环境内的变化。在互惠关联中，沃尔巴克氏体可能为宿主提供代谢物，或帮助维持宿主细胞的化学稳态。一些毒株可以通过操纵宿主生殖生物学快速侵入昆虫种群，同时还阻止病毒复制，允许它们用于节肢动物传播病毒的载体控制。的白纹伊蚊S-衍生菌株瓦特AlbB 在这方面很有希望。当转染黄热病蚊子，埃及伊蚊，w AlbB 在野生种群中达到高频率，并强烈抑制病毒传播。尽管它具有明显的潜力，但关于Wolbachia和宿主之间的分子相互作用，使其在病媒控制中的应用仍有很多未知。此外，迄今为止，大多数Wolbachia转染研究都集中在宿主效应上。在当前的研究中，我们使用细胞系模型来探索从Ae转染w AlbB的效果。albopictus到Ae。埃及. 使用 RNA 测序，我们表明与宿主-共生体相互作用相关的几个基因被转染下调，其中最大的下调是由原噬菌体相关基因表现出来的。

更新日期：2020-12-16

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