1. Batrachochytrium dendrobatidis (Bd) is a pathogenic fungus that has devastated amphibian populations globally by causing the disease chytridiomycosis. Batrachochytrium dendrobatidis is capable of infecting non‐amphibian hosts, such as crayfish, and has been detected on reptile and bird species. Given the taxonomic heterogeneity in the known hosts and vectors of Bd, it is likely that there is a diversity of undiscovered non‐amphibian hosts of the fungus.
2. Here, we investigated whether Bd could survive on freshwater snails (Physella acuta) and Cladophora algae. We exposed small and large snails (n = 15 snails/size category), Cladophora algae (n = 5), and artificial spring water controls (ASW; n = 5) to live Bd. We also maintained Bd‐free control snails (n = 5 snails/size category) in ASW. All treatments were maintained for 7 weeks at 18°C. Mortality was checked three times a week, snails were weighed every 2 weeks, and 7 weeks after exposure, the snails, algae, and water were tested for Bd using quantitative polymerase chain reaction.
3. We found that Bd did not grow on live snails, algae, or ASW long term. Additionally, live snails (n = 20) collected from Bd‐positive ponds in California were all negative for Bd, as well. Given that we found no Bd on the experimentally exposed or field swabbed snails, snails are probably not a reservoir host of Bd.
4. While negative results are often not published, Bd is one of the deadliest pathogens on earth; it is essential to know what is and is not capable of maintaining Bd for well‐designed disease models.

中文翻译：

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淡水蜗牛和绿藻类刺藻可能不是巴氏梭菌的寄主

1. 嗜盐杆菌（Batrachochytrium dendrobatidis，Bd）是一种致病性真菌，通过引起乳糜菌病而在全球范围内破坏了两栖动物种群。梭状芽孢杆菌能够感染非两栖动物宿主，例如小龙虾，并已在爬行动物和鸟类中发现。鉴于已知的Bd宿主和载体在分类学上的异质性，很可能存在多种未发现的真菌非两栖动物宿主。
2. 在这里，我们调查了Bd是否可以在淡水蜗牛（Physella acuta）和Cladophora藻类上存活。我们暴露了大大小小的蜗牛（n  = 15只蜗牛/大小类别），枝形藻（Cladophora藻类）（n  = 5）和人造泉水对照（ASW；n  = 5）来存活Bd。我们还在 ASW中维护了无Bd的控制蜗牛（n = 5蜗牛/大小类别）。所有处理均在18°C维持7周。每周检查3次死亡率，每2周对蜗牛称重一次，在暴露后7周，使用定量聚合酶链反应对蜗牛，藻类和水的Bd进行测试。
3. 我们发现Bd长期没有在活蜗牛，藻类或ASW上生长。此外， 从加利福尼亚的Bd阳性池塘收集的活蜗牛（n = 20）也均为Bd阴性。鉴于我们在实验暴露或田间擦拭的蜗牛中未发现Bd，因此蜗牛可能不是Bd的宿主。
4. 尽管负面结果通常没有公布，但Bd是地球上最致命的病原体之一。对于精心设计的疾病模型，了解什么是和不能够维持Bd至关重要。