Rice line 1892S is an elite thermo-sensitive genic male sterile (TGMS) line for two-line hybrid rice production. However, 1892S is susceptible to rice blast, bacterial blight and submergence. Here we reported the introduction of blast resistance (R) gene Pi9, bacterial blight R gene Xa21 and submergence tolerance gene Sub1A into 1892S genetic background through backcrossing and marker-assisted selection. The improved TGMS line 31892S and its hybrids conferred disease resistance to rice blast and bacterial blight, and showed submergence tolerance for over 14 d without significant loss of viability. The sterility- fertility conversion of 31892S was similar to that of 1892S. 31892S and its derived hybrid rice had similar agronomic traits and grain quality with 1892S and the control hybrid rice, respectively. The newly developed 31892S provided an improved TGMS line for two-line hybrid rice production with disease resistance to rice blast and bacterial blight, and submergence tolerance with no yield penalty or change in grain quality.

水稻品系1892S是用于两系杂交水稻生产的优良热敏基因雄性不育（TGMS）品系。但是，1892S易受稻瘟病，细菌性疫病和淹没的影响。在这里我们报告了稻瘟病抗性（R）基因Pi9，细菌白叶枯病R基因Xa21和淹没耐受基因Sub1A的引入。通过回交和标记辅助选择进入1892S的遗传背景。改良的TGMS品系31892S及其杂种赋予水稻稻瘟病和细菌性白叶枯病抗病性，并显示了超过14 d的淹没耐受性，而没有显着降低活力。31892S的不育－育性转化与1892S相似。31892S及其衍生的杂交稻的农艺性状和籽粒品质分别与1892S和对照杂交稻相似。新开发的31892S提供了改进的TGMS系，用于生产两系杂交水稻，具有抗稻瘟病和白叶枯病的病害，并且具有淹没耐受性，且不会增加产量或改变谷物品质。