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Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers
Animal Reproduction Science ( IF 2.2 ) Pub Date : 2020-12-16 , DOI: 10.1016/j.anireprosci.2020.106681
H P Dias 1 , R K Poole 2 , J P Albuquerque 1 , P H Dos Santos 3 , A C S Castilho 4 , K G Pohler 2 , J L M Vasconcelos 1
Affiliation  

The objective was to investigate effects of progesterone (P4) dose on abundance of luteinizing hormone receptor (LHCGR), aromatase (CYP19A1), 3β-hydroxysteroid dehydrogenase (HSD3B1), and other steroidogenic mRNA transcripts in granulosa cells from dominant follicles. Nellore heifers were assigned to one of six groups: new, first-use controlled internal drug release device (CIDR1) inserted for 5 days (Large-P4-dose-D5; n = 7) or 6 days (Large-P4-dose-D6; n = 8), prostaglandin (PG)F administered on D0 and 1 previously-used CIDR (CIDR3) inserted for 5 days (Small- P4-dose-D5; n = 8) or 6 days (Small-P4-dose-D6; n = 8), CIDR1 inserted on D0 and removed plus PGF on D5 (Large-P4-dose-proestrus (PE); n = 7), and CIDR3 and PGF on D0 and 1, CIDR3 removed plus PGF on D5 (Small-P4-dose-PE; n = 7). Duration of P4 treatment (D5 compared to D6) affected abundances of CYP19A1 mRNA transcripts, with there being greater abundances on D6 than D5 (P ≤ 0.05). Heifers treated with the large dose of P4 had a smaller dominant follicle, less serum and intra-follicular estradiol (E2) concentrations (P ≤ 0.05) and lesser LHCGR, CYP19A1, and HSD3B1 transcript abundances (P ≤ 0.05). Heifers treated to induce PE had a larger follicle diameter (P = 0.09), greater intra-follicular E2 concentrations and larger abundances of CYP19A1 mRNA transcript (P ≤ 0.05) than heifers of the D6 group. Overall, treatment with larger doses of P4 resulted in lesser abundances of LHCGR, HSD3B1, and CYP19A1 mRNA transcripts; thus, potentially leading to development of smaller dominant follicles and lesser E2 concentrations.



中文翻译:

同步治疗期间的黄体酮剂量改变了内洛尔小母牛颗粒细胞中促黄体激素受体和类固醇生成酶 mRNA 的丰度

目的是研究黄体酮 (P 4 ) 剂量对来自优势卵泡的颗粒细胞中黄体生成素受体 ( LHCGR )、芳香酶 ( CYP19A1 )、3β-羟基类固醇脱氢酶 ( HSD3B1 ) 和其他类固醇生成 mRNA 转录物丰度的影响。Nellore 小母牛被分配到六组之一:插入 5 天(大 P 4 -dose-D5;n = 7)或 6 天(大 P 4 -剂量-D6;n = 8),在 D0 给药的前列腺素 (PG)F 和 1 个先前使用的 CIDR (CIDR3) 插入 5 天(小-P 4 -剂量-D5 ;n = 8) 或 6 天(Small-P 4 -dose-D6;n = 8),在 D0 上插入 CIDR1 并在 D5 上移除加上 PGF (大-P 4 -dose-proestrus (PE);n = 7)和CIDR3和PGF 的D0和1,CIDR3除去加PGF 上D5(小-P 4 -dose-PE; ñ = 7)。P 4处理的持续时间(D5 与 D6 相比)影响CYP19A1 mRNA 转录本的丰度,D6 的丰度高于 D5(P ≤ 0.05)。用大剂量 P 4处理的小母牛具有较小的优势卵泡,较低的血清和卵泡内雌二醇 (E 2 ) 浓度 ( P ≤ 0.05) 和较低的LHCGRCYP19A1HSD3B1转录本丰度 ( P ≤ 0.05)。与 D6 组的小母牛相比,经处理诱导 PE 的小母牛具有更大的卵泡直径(P = 0.09)、更高的卵泡内 E 2浓度和更大的CYP19A1 mRNA 转录物丰度(P ≤ 0.05)。总体而言,用较大剂量的 P 4 处理导致LHCGRHSD3B1CYP19A1 的丰度降低mRNA 转录本;因此,可能导致较小的优势卵泡的发育和较低的 E 2浓度。

更新日期:2021-01-06
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