Interleukin-6 (IL-6) as a pro-inflammatory cytokine demonstrate a critical role in the inflammatory response. Especially, the high levels of IL-6 measured in plasma have been associated with pathological inflammation. In this report, new molecularly imprinting biosensor on graphene quantum dots (GQDs)/functionalized multi-walled carbon nanotubes (f-MWCNTs) composite were prepared for IL-6 protein detection. The structures of GQDs, f-MWCNTs and GQDs/f-MWCNTs composite were highlighted by scanning electron microscope (SEM), transmission electron microscopy (TEM), raman spectroscopy, UV–vis spectroscopy, fourier transform infrared spectroscopy (FTIR), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and X-ray diffraction (XRD) method. Surface morphology characterization shows the nanoporous cavities as an effective biosensing area. IL-6 protein imprinted electrode was prepared on GQDs/f-MWCNTs composite in the presence of 100.0 mM pyrrole containing 25.0 mM IL-6 protein. 0.01–2.0 pg ml⁻¹ and 0.0030 pg ml⁻¹ were found as linearity range and the detection limit (LOD) for analytical application in plasma samples. Finally, the validated biosensor was examined in terms of stability, repeatability and reproducibility.

白细胞介素6（IL-6）作为促炎细胞因子在炎症反应中显示出关键作用。特别地，血浆中测得的高水平的IL-6与病理性炎症有关。在本报告中，制备了新的分子印迹生物传感器在石墨烯量子点（GQDs）/功能化的多壁碳纳米管（f-MWCNTs）复合材料上用于IL-6蛋白检测。GQDs，f-MWCNTs和GQDs / f-MWCNTs复合材料的结构通过扫描电子显微镜（SEM），透射电子显微镜（TEM），拉曼光谱，UV-可见光谱，傅立叶变换红外光谱（FTIR），电化学阻抗来突出光谱法（EIS），循环伏安法（CV）和X射线衍射（XRD）方法。表面形态表征表明纳米孔腔是有效的生物传感区域。在含有25.0 mM IL-6蛋白的100.0 mM吡咯存在下，在GQDs / f-MWCNTs复合材料上制备了IL-6蛋白印迹电极。0.01–2.0 pg毫升^发现-1和0.0030 pg ml ^-1作为线性范围和血浆样品分析应用的检测极限（LOD）。最后，从稳定性，可重复性和可再现性方面对经过验证的生物传感器进行了检查。