Many growth factors have been paired with synthetic bone grafts to accelerate the healing process in vivo. Collagen has been particularly examined as a mediator of the enhancement of bone regeneration. This study investigated the new bone formation potential of micro–macroporous biphasic calcium phosphate (m-BCP), high porosity biphasic calcium phosphate (p-BCP), and collagen-coated p-BCP (cp-BCP) using a rabbit calvarial defect model. At 2 or 8 weeks after surgery, bone tissue was collected. The three-dimensional analysis of new bone formation using synchrotron radiation micro-computed tomography and histological study were conducted. The new bone formation values observed at 2 and 8 weeks in the negative control, m-BCP, p-BCP, and cp-BCP groups were 11.21 1.36 mm³, 21.75 1.18 mm³, 24.59 1.26 mm³, and 29.54 2.72 mm³, respectively, and 18.34 3.99 mm³, 32.27 3.78 mm³, 43.12 1.61 mm³, and 58.20 3.84 mm³, respectively. New bone formation was greatest in the cp-BCP group, while the amount of new bone at 8 weeks was higher than at 2 weeks in each group. The use of cp-BCP to enhance new bone formation during the healing period could improve bone regeneration.

许多生长因子已与合成骨移植物配对，以加速体内愈合过程。胶原蛋白已被特别检查为增强骨再生的介质。本研究使用兔颅骨缺损模型研究了微大孔双相磷酸钙 (m-BCP)、高孔隙率双相磷酸钙 (p-BCP) 和胶原蛋白涂层 p-BCP (cp-BCP) 的新骨形成潜力。 . 在手术后 2 或 8 周，收集骨组织。使用同步辐射微型计算机断层扫描和组织学研究对新骨形成进行了三维分析。阴性对照、m-BCP、p-BCP 和 cp-BCP 组在 2 周和 8 周观察到的新骨形成值分别为 11.21 1.36 mm ³、21.75 1.18 mm^3、24.59 1.26 mm ³和29.54 2.72 mm ³，分别为18.34 3.99 mm ³、32.27 3.78 mm ³、43.12 1.61 mm ³和58.20 3.84 mm ³。cp-BCP组的新骨形成最多，而各组8周时的新骨量均高于2周时。在愈合期间使用 cp-BCP 来增强新骨形成可以改善骨再生。