An ongoing challenge in drug delivery systems for a variety of medical applications, including cardiovascular diseases, is the delivery of multiple drugs to address numerous phases of a treatment or healing process. Therefore, an extended dual drug delivery system (DDDS) based on our previously reported cardiac DDDS was generated. Here we use the polymer poly(L-lactide) (PLLA) as drug carrier with the cytostatic drug Paclitaxel (PTX) and the endothelial cell proliferation enhancing growth factor, human vascular endothelial growth factor (VEGF), to overcome typical in-stent restenosis complications. We succeeded in using one solution to generate two separate DDDS via spray coating (film) and electrospinning (nonwoven) with the same content of PTX and the same post processing for VEGF immobilisation. Both processes are suitable as coating techniques for implants. The contact angle analysis revealed differences between films and nonwovens. Whereas, the morphological analysis demonstrated nearly no changes occurred after immobilisation of both drugs. Glass transition temperatures (T_g ) and degree of crystallinity (χ) show only minor changes. The amount of immobilised VEGF on nonwovens was over 300% higher compared to the films. Also, the nonwovens revealed a much faster and over three times higher PTX release over 70 d compared to the films. The almost equal physical properties of nonwovens and films allow the comparison of both DDDS independently of their fabrication process. Both films and nonwovens have significantly increased in vitro cell viability for human umbilical vein endothelial cells (EA.hy926) with dual loaded PTX and VEGF compared to PTX-only loaded samples.

用于各种医疗应用（包括心血管疾病）的药物输送系统面临的一个持续挑战是输送多种药物以解决治疗或愈合过程的多个阶段。因此，生成了基于我们先前报道的心脏 DDDS 的扩展双药物输送系统 (DDDS)。在这里，我们使用聚合物聚（L-丙交酯）（PLLA）作为药物载体与细胞抑制药物紫杉醇（PTX）和内皮细胞增殖促进生长因子，人血管内皮生长因子（VEGF），以克服典型的支架内再狭窄并发症。我们成功地使用一种解决方案通过喷涂（薄膜）和静电纺丝（非织造布）产生了两个单独的 DDDS，具有相同的 PTX 含量和相同的 VEGF 固定后处理。这两种工艺都适合作为植入物的涂层技术。接触角分析揭示了薄膜和非织造布之间的差异。然而，形态分析表明两种药物固定后几乎没有发生变化。玻璃化转变温度 (T _g ) 和结晶度 (χ) 仅显示很小的变化。与薄膜相比，非织造布上固定化 VEGF 的量高出 300% 以上。此外，与薄膜相比，非织造布在 70 天内的 PTX 释放速度更快，PTX 释放速度高出三倍以上。无纺布和薄膜的物理性能几乎相同，因此可以独立于它们的制造工艺对两种 DDDS 进行比较。与仅加载 PTX 的样品相比，薄膜和非织造布均显着提高了双加载 PTX 和 VEGF 的人脐静脉内皮细胞 (EA.hy926) 的体外细胞活力。