Antimicrobial peptides (AMPs) are considered as novel potential alternatives to antibiotics against increasing number of multi drug resistant (MDR) pathogens. Although AMPs have shown strong antimicrobial activity against gram-negative or gram-positive microorganisms, AMP conjugated biomaterials that are effective against MDR microorganisms are yet to be developed. Herein, the potential use of (RWRWRWRW)-NH2 (AMP-1) and KRFRIRVRV-NH2 (AMP-2) peptide conjugated electrospun polylactic–co-glycolic-acid (PLGA) nanofibers (NFs) fabricated and their antimicrobial effect by themselves and in their dual combination (1:1) were evaluated on P. aeruginosa and methicillin-resistant S. aureus (MRSA). Those AMP conjugated NFs did not inhibit proliferation of keratinocytes. These results suggest that AMP conjugated NF, which has multiple biological activities, would be a promising candidate as a wound dressing material.

抗菌肽 (AMP) 被认为是抗生素的新型潜在替代品，可对抗越来越多的多药耐药 (MDR) 病原体。尽管 AMP 对革兰氏阴性或革兰氏阳性微生物表现出很强的抗菌活性，但对 MDR 微生物有效的 AMP 缀合生物材料仍有待开发。在此，(RWRWRWRW)-NH2 (AMP-1) 和 KRFRIRVRV-NH2 (AMP-2) 肽共轭电纺聚乳酸-共-乙醇酸 (PLGA) 纳米纤维 (NFs) 的潜在用途及其自身的抗菌作用和在他们的双重组合 (1:1) 中对铜绿假单胞菌和耐甲氧西林金黄色葡萄球菌进行了评估(MRSA)。那些 AMP 结合的 NF 不抑制角质形成细胞的增殖。这些结果表明，具有多种生物活性的 AMP 共轭 NF 将是一种很有前途的伤口敷料材料。