A role for incisional biopsy in preoperative prognostication is increasingly being advocated in oral tongue squamous cell carcinomas (OTSCC). Biopsies at two locations were compared, and prognostic factors in biopsies and their corresponding resections were evaluated. A total of 138 OTSCC biopsy slides from Finland and Saudi Arabia were compared for size (horizontal and vertical) and invasive front. The Finnish cases were assessed for tumor stroma ratio (TSR) and tumor‐infiltrating lymphocytes (TILs) using light microscopy and digital image analysis assessment and compared. Furthermore, TSR, TILs, and previously analyzed budding and depth of invasion (BD) score in biopsies were compared with their evaluation in the corresponding resections. Fifty‐nine percent of Finnish and 42% of Saudi Arabian biopsies were ≥ 5 mm deep, while 98% of Saudi Arabian and 76% of Finnish biopsies were ≥ 5 mm wide. Assessment of invasion front was possible in 72% of Finnish in comparison with 40% of Saudi Arabian biopsies. There was 86.8% agreement between TSR and 75% agreement between TIL evaluation using light microscopy and digital assessment. Significant agreement was obtained on comparing the TSR (p = 0.04) and BD (p < 0.001) values in biopsies and resections. Biopsies of ≥ 5 mm depth from representative OTSCC areas are essential for prognostic information. Clinical pathologists are advised to assess BD score and TSR for prognostic features in such biopsies.

中文翻译：

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活检质量对于口腔癌的术前预后至关重要

在口腔舌鳞状细胞癌（OTSCC）中，越来越多地提倡切口活检在术前预后中的作用。比较了两个位置的活检，并评估了活检及其相应切除的预后因素。比较了来自芬兰和沙特阿拉伯的总共138份OTSCC活检玻片的大小（水平和垂直）和侵袭性前沿。使用光学显微镜和数字图像分析评估方法评估并比较芬兰病例的肿瘤基质比例（TSR）和肿瘤浸润淋巴细胞（TIL）。此外，将TSR，TIL和先前分析的活检中的萌芽和浸润深度（BD）得分与相应切除中的评估结果进行了比较。59％的芬兰人和42％的沙特阿拉伯活组织检查的深度≥5 mm，而98％的沙特阿拉伯和76％的芬兰活检宽度≥5 mm。与40％的沙特阿拉伯活检相比，有72％的芬兰人可以评估侵袭锋线。使用光学显微镜和数字评估的TIL评估之间TSR的一致性为86.8％，TIL评估的一致性为75％。在活检和切除术中比较TSR（p = 0.04）和BD（p <0.001）值时，取得了显着共识。距代表性OTSCC区域的深度≥5 mm的活检对于预后信息至关重要。建议临床病理学家评估BD评分和TSR在此类活检中的预后特征。TSR之间的一致性为8％，而使用光学显微镜和数字评估的TIL评估之间的一致性为75％。在活检和切除术中比较TSR（p = 0.04）和BD（p <0.001）值时，取得了显着共识。距代表性OTSCC区域的深度≥5 mm的活检对于预后信息至关重要。建议临床病理学家评估BD评分和TSR在此类活检中的预后特征。TSR之间的一致性为8％，而使用光学显微镜和数字评估的TIL评估之间的一致性为75％。在活检和切除术中比较TSR（p = 0.04）和BD（p <0.001）值时，取得了显着共识。距代表性OTSCC区域的深度≥5 mm的活检对于预后信息至关重要。建议临床病理学家评估BD评分和TSR在此类活检中的预后特征。