Because of logistics and cost constraints, monitoring of the compliance to antimalarial chemoprophylaxis by the quantitation of drugs in biological samples is not a simple operation on the field. Indeed, analytical devices are fragile to transport and must be used in a perfectly controlled environment. This is also the case for reagents and supplies, and the waste management is constraining. Thus, samples should be repatriated. They should be frozen after collection and transported with no rupture in the cold chain. This is crucial to generate available and interpretable data but often without any difficulties. Hence, to propose an alternative solution easier to implement, a quantitation method of determining doxycycline in urine has been validated using a volumetric absorptive microsampling (VAMS^®) device. As blotting paper, the device is dried after collection and transferred at room temperature, but contrarily to dried spot, the collection volume is perfectly repeatable. Analysis of VAMS^® was performed with a high-performance liquid chromatography coupled to a mass spectrometer. The chromatographic separation was achieved on a core-shell C18 column. The mean extraction recovery was 109% (mean RSD, 5.4%, n = 6) for doxycycline and 102% (mean RSD, 7.0%) for the internal standard. No matrix effect has been shown. Within-run as within-day precision and accuracy were, respectively, below 14% and ranged from 96 to 106%. The signal/concentration ratio was studied in the 0.25–50 µg/mL range, and recoveries from back-calculated concentrations were in the 96–105% range (RSD < 11.0%). The RSD on slope was 10%. To achieve the validation, this new quantitation method was applied to real samples. In parallel, samples were analyzed directly after a simple dilution. No statistical difference was observed, confirming that the use of VAMS^® is an excellent alternative device to monitor the doxycycline compliance.

中文翻译：

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经验证的容积吸收微采样-液相色谱串联质谱法定量尿液中的强力霉素水平：一种监测疟疾化学预防依从性的应用

由于物流和成本的限制，在现场通过定量生物样品中的药物来监测对抗疟疾化学预防的依从性并不是一个简单的操作。确实，分析设备易碎，必须在完全受控的环境中使用。试剂和耗材也是如此，废物管理受到限制。因此，应将样品归还。收集后应将其冷冻，并在冷链中无破裂地运输。这对于生成可用的和可解释的数据至关重要，但通常没有任何困难。因此，提出一种替代的解决方案更易于实现，确定在尿多西环素的定量方法已经使用体积吸收微量（VAMS被验证^®）设备。作为吸水纸，该装置在收集后干燥并在室温下转移，但与干燥点相反，收集体积可完全重复。VAMS分析^®用耦合到质谱仪高效液相色谱法进行的。色谱分离在核-壳C18柱上完成。 多西环素的平均提取回收率为109％（平均RSD，5.4％，n = 6），内标的平均提取回收率为102％（平均RSD，7.0％）。没有显示基质效应。运行内的日内精度和准确度分别低于14％，范围为96％至106％。在0.25–50 µ中研究了信号/浓度比 g / mL范围，反算浓度的回收率在96–105％范围内（RSD <11.0％）。斜率的RSD为10％。为了实现验证，将这种新的定量方法应用于实际样品。平行地，在简单稀释后直接分析样品。未观察到统计学差异，证实了使用VAMS的^®是一个很好的替代设备来监视多西环素顺应性。