Chemical investigation of the aerial parts (except fruits) of the medicinal, hallucinogen and toxic plant Datura innoxia Mill. [Solanaceae] led to the isolation of the new withanolide, dinnoxolide A ( 1 ), along with the known compounds 21,27-dihydroxy-1-oxowitha-2,5,24-trienolide ( 2 ), daturamalakin B ( 3 ) and withametelin ( 4 ). Their structures were established by analysis of their spectroscopic data, including 1D and 2D NMR experiments and MS. Compounds 2 and 3 were isolated as natural products for the first time and the name dinnoxolide B was given to compound 2 . The four withanolides showed in vitro cytotoxic activity against U251 (glioblastoma) and SK-LU-1 (lung adenocarcinoma) human cancer cell lines, with IC 50 values ranging from 1.2 to 19.6 µM, and also against the noncancerous monkey kidney cell line (COS-7), with IC 50 values ranging from 5.0 to 19.7 µM. Compound 4 was two times more active than the reference compound, etoposide, against lung adenocarcinoma cells.

中文翻译：

---

来自曼陀罗的细胞毒性 withanolides

药用、致幻剂和有毒植物曼陀罗地上部分（果实除外）的化学研究。[茄科] 分离出了新的山梨醇内酯，dinnoxolide A ( 1 )，以及已知的化合物 21,27-dihydroxy-1-oxowitha-2,5,24-trienolide (2)、daturamalakin B (3) 和withametelin (4)。它们的结构是通过分析光谱数据确定的，包括 1D 和 2D NMR 实验和 MS。化合物2和3首次作为天然产物分离出来，化合物2被命名为dinnoxolide B。四种 withanolides 对 U251（胶质母细胞瘤）和 SK-LU-1（肺腺癌）人癌细胞系显示出体外细胞毒活性，IC 50 值范围为 1.2 至 19.6 µM，也对非癌猴肾细胞系（COS） -7), IC 50 值范围为 5.0 至 19.7 µM。化合物 4 对肺腺癌细胞的活性是参考化合物依托泊苷的两倍。