Cerium Oxide Nanoparticle-Loaded Gelatin Methacryloyl Hydrogel Wound-Healing Patch with Free Radical Scavenging Activity,ACS Biomaterials Science & Engineering

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Cerium Oxide Nanoparticle-Loaded Gelatin Methacryloyl Hydrogel Wound-Healing Patch with Free Radical Scavenging Activity
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-12-14 , DOI: 10.1021/acsbiomaterials.0c01138
Robin Augustine _{1,

2} , Alap Ali Zahid _{1,

2} , Anwarul Hasan _{1,

2} , Yogesh Bharat Dalvi ₃ , Jessiya Jacob _{3,

4}

Affiliation

Nonhealing wounds in diabetic patients are a critical challenge, which often cause amputation and mortality. High levels of oxidative stress and aberrations in antioxidant defense mechanisms increase the adverse manifestations of diabetes mellitus. In this study, we developed a biodegradable gelatin methacryloyl (GelMA) hydrogel patch incorporated with cerium oxide nanoparticles (CONPs) for promoting diabetic wound healing. The patches were thoroughly characterized for the morphology, physicomechanical properties, free radical scavenging activity, in vitro cell proliferation, and in vivo diabetic wound healing activity. Highly porous and biodegradable patches showed excellent exudate uptake capacity as evident from the many-fold weight gain (400–700 times) when placed in aqueous medium. Results of free radical scavenging assays clearly indicated that the patches loaded with 1–4% w/w CONPs could effectively inactivate experimentally generated free radicals. Obtained results of in vitro cell culture studies clearly indicated that CONP-incorporated patches could favor the proliferation of skin-associated cells such as keratinocytes and fibroblasts. Results of the wound healing study showed that 1% w/w CONP-loaded patches could effectively improve the healing of wounds in diabetic rats. Overall results indicate that CONP-loaded GelMA hydrogels are highly promising materials for developing clinically relevant patches for treating diabetic wounds.

中文翻译：

具有自由基清除活性的氧化铈纳米粒子明胶甲基丙烯酰水凝胶伤口愈合贴剂

糖尿病患者的伤口无法愈合是一个严峻的挑战，通常会导致截肢和死亡。高水平的氧化应激和抗氧化防御机制中的畸变增加了糖尿病的不良表现。在这项研究中，我们开发了可生物降解的明胶甲基丙烯酰（GelMA）水凝胶贴剂，与氧化铈纳米颗粒（CONP）结合使用，可促进糖尿病伤口的愈合。对贴剂的形态，物理力学性能，自由基清除活性，体外细胞增殖和体内进行了全面表征糖尿病伤口的愈合活动。高度多孔且可生物降解的贴剂显示出优异的渗出液吸收能力，这可以从放置在水性介质中的体重增加几倍（400-700倍）中看出。自由基清除试验的结果清楚地表明，装有1-4％w / w CONP的贴剂可以有效地灭活实验产生的自由基。取得体外结果细胞培养研究清楚地表明，结合CONP的贴剂可能促进皮肤相关细胞（例如角质形成细胞和成纤维细胞）的增殖。伤口愈合研究的结果表明，载有1％w / w CONP的贴剂可以有效改善糖尿病大鼠伤口的愈合。总体结果表明，装载CONP的GelMA水凝胶是开发具有临床意义的用于治疗糖尿病伤口的贴剂的极有前途的材料。

更新日期：2021-01-11

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