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Regulation of Krüppel homolog 1 expression by photoperiod in the bean bug, Riptortus pedestris
Physiological Entomology ( IF 1.6 ) Pub Date : 2020-12-15 , DOI: 10.1111/phen.12347 Li Dong 1 , Hiroko Udaka 1 , Hideharu Numata 1 , Chihiro Ito 1
Physiological Entomology ( IF 1.6 ) Pub Date : 2020-12-15 , DOI: 10.1111/phen.12347 Li Dong 1 , Hiroko Udaka 1 , Hideharu Numata 1 , Chihiro Ito 1
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In Riptortus pedestris (Hemiptera, Alydidae), adult females develop their ovaries under long‐day conditions (LD), but enter reproductive diapause with suppression of ovarian development under short‐day conditions (SD). This photoperiodic response is regulated by juvenile hormone (JH). Krüppel homolog 1 (Kr‐h1) encodes a JH‐inducible transcriptional factor and plays a pivotal role for reproduction in some insects. In the present study, we molecularly characterized Kr‐h1 and investigated Kr‐h1 expression patterns under SD and LD in R. pedestris. The open reading frame of Kr‐h1 was 1320 bp encoding a protein of 440 amino acids. The deduced protein contained eight conserved C2H2‐type zinc finger domains. The expression levels of Kr‐h1 increased from 5 days and peaked at 7 days after transfer from SD to LD. By contrast, Kr‐h1 was constantly expressed at a low level under SD. Topical application of a JH analogue induced ovarian development and Kr‐h1 expression even under SD, suggesting that Kr‐h1 is a JH‐inducible gene in R. pedestris. Then, we examined the effect of knockdown of circadian clock genes Clock (Clk) and mammalian‐type cryptochrome (cry‐m) by RNA interference (RNAi) on the ovarian development and Kr‐h1 expression. Clk RNAi prevented ovarian development and decreased the Kr‐h1 expression under LD, whereas cry‐m RNAi induced ovarian development and Kr‐h1 expression under SD. These results imply that JH secretion is prevented by Clk RNAi under LD and induced by cry‐m RNAi under SD. From these results, we suggest that Kr‐h1 expression is JH dependent and closely correlates with photoperiodic regulation of ovarian development.
中文翻译：
菜豆臭虫Riptortus pedestris对光周期Krüppel同源物1表达的调节
在Riptortus pedestris（Hemiptera，Alydidae）中，成年雌性在长日照条件下（LD）发育卵巢，但在短日照条件下（SD）进入生殖滞育状态，抑制卵巢发育。这种光周期反应受少年激素（JH）的调节。Krüppel同系物1（Kr-h1）编码JH诱导型转录因子，在某些昆虫的繁殖中起关键作用。在本研究中，我们分子表征的Kr-H1和研究的Kr-H1在SD和LD下表达模式[R 。pedestris。Kr-h1的开放阅读框cDNA是1320bp，编码440个氨基酸的蛋白质。推导的蛋白质包含八个保守的C 2 H 2型锌指结构域。从SD转移到LD后，Kr-h1的表达水平从5天开始上升，并在7天达到峰值。相比之下，在SD下Kr-h1经常以低水平表达。JH类似物的局部应用即使在SD下也可诱导卵巢发育和Kr-h1表达，这表明Kr-h1是R中的JH诱导基因。pedestris。然后，我们研究了敲除昼夜节律时钟基因Clock（Clk）和RNA干扰（RNAi）对卵巢发育和Kr-h1表达的哺乳动物型隐花色素（cry-m）。Clk RNAi可阻止卵巢发育并降低LD下Kr-h1的表达，而cry-m RNAi可诱导卵巢发育和SD下Kr-h1的表达。这些结果意味着，JH的分泌是由防止Clk时的RNAi下LD和诱导一声-米的RNAi下SD。根据这些结果，我们认为Kr-h1表达是JH依赖性的，并且与卵巢发育的光周期调节密切相关。
更新日期：2021-02-09
中文翻译：
菜豆臭虫Riptortus pedestris对光周期Krüppel同源物1表达的调节
在Riptortus pedestris（Hemiptera，Alydidae）中，成年雌性在长日照条件下（LD）发育卵巢，但在短日照条件下（SD）进入生殖滞育状态，抑制卵巢发育。这种光周期反应受少年激素（JH）的调节。Krüppel同系物1（Kr-h1）编码JH诱导型转录因子，在某些昆虫的繁殖中起关键作用。在本研究中，我们分子表征的Kr-H1和研究的Kr-H1在SD和LD下表达模式[R 。pedestris。Kr-h1的开放阅读框cDNA是1320bp，编码440个氨基酸的蛋白质。推导的蛋白质包含八个保守的C 2 H 2型锌指结构域。从SD转移到LD后，Kr-h1的表达水平从5天开始上升，并在7天达到峰值。相比之下，在SD下Kr-h1经常以低水平表达。JH类似物的局部应用即使在SD下也可诱导卵巢发育和Kr-h1表达，这表明Kr-h1是R中的JH诱导基因。pedestris。然后，我们研究了敲除昼夜节律时钟基因Clock（Clk）和RNA干扰（RNAi）对卵巢发育和Kr-h1表达的哺乳动物型隐花色素（cry-m）。Clk RNAi可阻止卵巢发育并降低LD下Kr-h1的表达，而cry-m RNAi可诱导卵巢发育和SD下Kr-h1的表达。这些结果意味着，JH的分泌是由防止Clk时的RNAi下LD和诱导一声-米的RNAi下SD。根据这些结果，我们认为Kr-h1表达是JH依赖性的，并且与卵巢发育的光周期调节密切相关。
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