Effects of Pergularia tomentosa active fraction on the oogenesis of the desert locust, Schistocerca gregaria: ovarian biochemical composition, and effects on the ecdysteroid pathway,Physiological Entomology

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Effects of Pergularia tomentosa active fraction on the oogenesis of the desert locust, Schistocerca gregaria: ovarian biochemical composition, and effects on the ecdysteroid pathway
Physiological Entomology ( IF 1.6 ) Pub Date : 2020-12-14 , DOI: 10.1111/phen.12346
Meriam Miladi ₁ , Khemais Abdellaoui ₁ , Elisabeth Marchal ₂ , Cynthia Lenaerts ₂ , Amel Ben Hamouda ₁ , Meriem M'saad Guerfali ₃ , Monia Ben Halima Kamel ₁ , Jozef Vanden Broeck ₂

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Fractionation of the methanolic extract of Pergularia tomentosa, using column chromatography, resulted in the separation of 10 fractions and only the active one (that gave mortality >70%) was kept and denoted the active fraction (AFPt). Adult female Schistocerca gregaria were treated with two concentrations of AFPt, 0.24% and 0.96%, to investigate the effect on the female reproductive potential. Both concentrations of AFPt significantly increased the duration between adult emergence and first oviposition, as well as affected fecundity and fertility. Furthermore, the effects of AFPt on vitellogenesis and ecdysteroid titres were examined. Biochemical analyses revealed a significant decrease in protein, lipid and carbohydrate contents in ovaries. This study also showed that AFPt induced a significant decrease in the ovarian ecdysteroids levels, as well as the haemolymph ecdysteroid titres. The transcript levels of the ecdysteroid synthesis enzymes Spook and Shadow were significantly decreased when treated with the highest concentration of AFPt, while transcript levels of the ecdysteroid synthesis enzymes Phantom and Disembodied increased significantly in animals treated with AFPt. Treatment with AFPt also resulted in a significantly higher transcript level of both the ecdysone receptor (EcR) and its partner ultraspiracle/retinoid‐X‐receptor (USP/RXR).

中文翻译：

毛白粉刺活性级分对沙漠蝗，卵裂血吸虫的产卵作用：卵巢生化成分及其对蜕皮甾体途径的影响

使用柱色谱法分离毛白顶草的甲醇提取物，分离出10个馏分，仅保留了活性馏分（死亡率> 70％），并将其表示为活性馏分（AFPt）。成年雌性血吸虫用两种浓度的AFPt（0.24％和0.96％）进行处理，以研究其对女性生殖潜能的影响。两种浓度的AFPt均显着增加了成年至首次产卵之间的持续时间，并影响了生育力和繁殖力。此外，检查了AFPt对卵黄发生和蜕皮甾体滴度的影响。生化分析显示卵巢中蛋白质，脂质和碳水化合物含量显着降低。该研究还表明，AFPt诱导卵巢蜕皮甾体水平以及血淋巴蜕皮甾体滴度显着降低。蜕皮类固醇合成酶Spook and Shadow的转录水平当与AFPt的最高浓度处理过显著下降，而蜕皮甾类合成的酶的转录水平幻影和无实体与AFPt处理的动物显著增加。治疗AFPt也导致两个蜕皮激素受体（的显著较高的转录水平的EcR）及其合作伙伴超气门/维甲酸X受体（USP / RXR）。

更新日期：2021-02-09

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