Structure-based molecular networking is useful as a dereplication strategy to identify known molecules, unknown close analogues, or compound families. On the other hand, the ligand fishing assay is a remarkable alternative to accelerate the screening process and to overcome the drawbacks of laborious experiments usually adopted in natural product research. The combination of these approaches contributes to high productivity in disclosing active metabolites and a decrease in lead time identification. To provide a valuable data base for the alkaloids of A. salzmannii bark herein we disclose thirty-one isoquinoline alkaloids including benzyltetrahydroisoquinolines, aporphines, proaporphines, and protoberberines. Among these, twenty-six have not been described for A. salzmannii including the unprecedented alkaloid N,O-dimethylcoclaurine N-oxide. In addition, norcoclaurine (1), norreticuline (13), N,O-dimethylcoclaurine N-oxide (15), and N-acetylasimilobine (24) are now reported for the first time as ligand for acetylcholinesterase.

基于结构的分子网络可用作确定已知分子，未知紧密类似物或化合物家族的重复复制策略。另一方面，配体捕捞测定法是加快筛选过程并克服天然产物研究中通常采用的费力实验的弊端的显着替代方法。这些方法的组合有助于揭示活性代谢物的高生产率，并缩短交货时间。为了为本文的萨尔茨曼氏菌树皮的生物碱提供有价值的数据库，我们公开了三十一种异喹啉生物碱，包括苄基四氢异喹啉，磷灰石，原卟啉和原小ber碱。在这些中，没有描述用于萨尔茨曼氏菌的二十六个包括史无前例的生物碱N，O-二甲基coclaurine N-氧化物。另外，现在首次报道了降丁草碱（1），去甲肾上腺素（13），N，O-二甲基甲草碱N-氧化物（15）和N-乙酰基亚氨倍碱（24）作为乙酰胆碱酯酶的配体。