The effect of alginate encapsulation on the viability of Lactobacillus rhamnosus GG in provitamin A cassava hydrolysate used as a carrier during processing, storage, in vitro and in vivo gastro-intestinal conditions was assessed. Several studies in the literature have reported the viability of probiotics in non-dairy food matrices such as fruits and vegetables. However, there is need for further studies exploring the maintenance of probiotics in other non-dairy options and to evaluate the resistance of probiotics during passage through the gastro-intestinal tract. Lactobacillus rhamnosus GG (LGG) cells were microencapsulated in alginate beads (using emulsion and extrusion techniques), characterized and inoculated into provitamin A cassava starch hydrolysate. The survival/viability rates of LGG in provitamin A hydrolysate during storage (4 °C, 90 days), also in gastric and intestinal conditions (in vitro and in vivo) were measured. Free LGG cells in provitamin A cassava hydrolysates decreased rapidly in viability within the first 30 days of storage whereas encapsulated cells showed a gradual but insignificant decrease in viability. LGG cells maintained viability above 5 Log CFU/mL in hydrolysates with free LGG, and above 7 Log CFU/g in hydrolysates with encapsulated LGG by the end of 60 days of storage. Encapsulated LGG had 77.4 and 8.5% survival after 120 min in simulated gastric and intestinal juices, respectively. Growth direction index expanded up to 1.66 for LGG but decreased below −0.05 for total aerobes and Enterobacteriaceae in faeces of rats administered hydrolysate with encapsulated or free LGG by day 30. Encapsulated LGG showed better resistance during transit through gastrointestinal conditions.

藻酸盐包封的对的生存力的影响鼠李糖乳杆菌GG处理，存储，在用作载体中维生素A木薯水解产物在 体外和在 体内胃肠条件进行了评估。文献中的几项研究报告了益生菌在非乳制品食品基质（例如水果和蔬菜）中的生存能力。然而，需要进一步的研究探索其他非乳制品中益生菌的维持，并评估益生菌在胃肠道中的抵抗力。鼠李糖乳杆菌GG（LGG将细胞微囊化在藻酸盐珠粒中（使用乳液和挤出技术），进行表征并接种到原维生素A木薯淀粉水解物中。的存活/活力率LGG在维生素A的水解产物在储存期间（4℃，90天），同样在胃和肠的条件（在 体外和在 体内测定）。原维生素A木薯水解产物中的游离LGG细胞在储存的前30天内活力迅速降低，而被包囊的细胞则显示出活力逐渐降低但不明显。LGG细胞在含有游离LGG的水解产物中的活力保持在5 Log CFU / mL以上，到60天储存结束时，含封装的LGG的水解产物中的CLog / g超过7 Log CFU / g 。在模拟的胃液和肠液中，封装的LGG在120分钟后的存活率分别为77.4和8.5％。在第30天时，将水解后的含密封或游离LGG的大鼠粪便中LGG的生长方向指数扩大至1.66，但总需氧菌和肠杆菌科的生长方向指数降低至-0.05以下。密封LGG在通过胃肠道条件下表现出更好的抵抗力。