Extracellular matrix (ECM) scaffolds are extensively used in tissue engineering studies and numerous clinical applications for tissue and organ reconstructions. Due to the global severe shortage of human tissues and organs, xenogeneic biomaterials are a common source for human tissue engineering and regenerative medicine applications. Traditional methods for decellularization often disrupt the 3D architecture and damage the structural integrity of the ECM scaffold. To efficiently obtain natural ECM scaffolds from animal tissues and organs with intact architecture, we have developed a platform decellularization process using supercritical CO₂ and tested its potential application in tissue engineering. A combination of human mesenchymal stem cells with a decellularized dermal matrix scaffold allowed complete regeneration of skin structure in a porcine full-thickness wound model.

中文翻译：

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用于组织工程的脱细胞组织和器官支架的制备和表征的协议。

细胞外基质 (ECM) 支架广泛用于组织工程研究和组织和器官重建的众多临床应用。由于全球人体组织器官严重短缺，异种生物材料是人体组织工程和再生医学应用的常见来源。传统的去细胞方法通常会破坏 3D 结构并破坏 ECM 支架的结构完整性。为了从具有完整结构的动物组织和器官中有效地获得天然 ECM 支架，我们开发了一种使用超临界 CO ₂的平台脱细胞过程并测试了其在组织工程中的潜在应用。人类间充质干细胞与脱细胞真皮基质支架的组合允许在猪全层伤口模型中完全再生皮肤结构。