Being able to vizualize a pathogen at a site of interaction with a host is an aesthetically appealing idea and the resulting images can be both informative as well as enjoyable to view. Moreover, the approaches used to derive these images can be powerful in terms of offering data unobtainable by other methods. In this article, we review three primary modalities for live imaging Borrelia spirochetes: whole animal imaging, intravital microscopy and live cell imaging. Each method has strengths and weaknesses, which we review, as well as specific purposes for which they are optimally utilized. Live imaging borriliae is a relatively recent development and there was a need of a review to cover the area. Here, in addition to the methods themselves, we also review areas of spirochete biology that have been significantly impacted by live imaging and present a collection of images associated with the forward motion in the field driven by imaging studies.

中文翻译：

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 实时成像。


能够在与宿主相互作用的部位可视化病原体是一个具有美学吸引力的想法，并且生成的图像既可以提供丰富的信息，又可以令人赏心悦目。此外，用于导出这些图像的方法在提供其他方法无法获得的数据方面非常强大。在本文中，我们回顾了疏螺旋体螺旋体实时成像的三种主要方式：整体动物成像、活体显微镜和活细胞成像。每种方法都有其优点和缺点，我们会对其进行审查，以及最佳利用它们的具体目的。实时成像螺旋体是一个相对较新的发展，需要进行审查以覆盖该领域。在这里，除了方法本身之外，我们还回顾了受实时成像显着影响的螺旋体生物学领域，并呈现了与成像研究驱动的领域向前运动相关的图像集合。