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Simultaneous extraction and analysis of clozapine and lorazepam from human plasma using dual solvent-stir bar microextraction with different acceptor phases followed by high-performance liquid chromatography ultra-violet detection
Analytical Methods ( IF 2.7 ) Pub Date : 2020-11-25 , DOI: 10.1039/d0ay01431a Mahsa Sheikh 1, 2, 3, 4, 5 , Mohammad Reza Hadjmohammadi 1, 2, 3, 4, 5 , Mohammad Hossein Fatemi 1, 2, 3, 4, 5
Analytical Methods ( IF 2.7 ) Pub Date : 2020-11-25 , DOI: 10.1039/d0ay01431a Mahsa Sheikh 1, 2, 3, 4, 5 , Mohammad Reza Hadjmohammadi 1, 2, 3, 4, 5 , Mohammad Hossein Fatemi 1, 2, 3, 4, 5
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A new design of dual solvent stir bar microextraction (DSSBME) was developed and combined with HPLC-UV for the simultaneous extraction of clozapine (CLZ) and lorazepam (LRP) from human plasma with different acceptor phases. Two short hollow fibers immobilized with an organic extraction solvent were used as the solvent bars for microextraction of CLZ and LRP from the sample solution. The solvent bars were fixed with a staple pin which served as the stirrer. The target analytes were simultaneously and selectively extracted from the sample solution into their corresponding solvent bar. Extraction parameters such as organic solvent type, pH of the sample solution, the acceptor phase concentration, salt incorporation into the solution, stirring rate, and extraction time were optimized to achieve the best extraction results. Under the optimum conditions (1-undecanol as extraction solvent, pH of sample solution = 9.0, 10% w/v NaCl, concentration of HCl = 10 mM, concentration of NaOH = 100 mM, stirring rate of 1400 rpm and extraction time of 30 min at ambient temperature) the limit of detection for CLZ was 0.4 ng mL−1 and for LRP it was 1.1 ng mL−1. The linear range for CLZ was 1.3–1000.0 ng mL−1 (R2 = 0.9991) and for LRP it was 3.6–800.0 ng mL−1 (R2 = 0.9993). Extraction recovery and the enrichment factor for CLZ were 95.4% and 343 and for LRP they were 74.3% and 263, respectively. Finally, the method developed was successfully applied for the simultaneous determination of CLZ and LRP in human plasma samples.
中文翻译：
使用具有不同受体相的双溶剂搅拌棒微萃取同时进行高效液相色谱紫外检测同时从人血浆中提取和分析氯氮平和劳拉西m
开发了一种新的双溶剂搅拌棒微萃取（DSSBME）设计，并与HPLC-UV结合使用，可从具有不同受体相的人血浆中同时提取氯氮平（CLZ）和劳拉西m（LRP）。将固定有有机萃取溶剂的两条短中空纤维用作从样品溶液中微萃取CLZ和LRP的溶剂棒。用用作搅拌器的订书钉固定溶剂棒。同时将目标分析物从样品溶液中选择性提取到其相应的溶剂棒中。优化了萃取参数，如有机溶剂类型，样品溶液的pH，受体相浓度，溶液中的盐掺入量，搅拌速率和萃取时间，以实现最佳萃取效果。-1和LRP为1.1 ng mL -1。CLZ的线性范围为1.3–1000.0 ng mL -1（ R 2 = 0.9991），LRP的线性范围为3.6–800.0 ng mL -1（ R 2 = 0.9993）。CLZ的提取回收率和富集因子分别为95.4％和343，LRP的提取回收率和富集因子分别为74.3％和263。最终，开发的方法成功地用于人血浆样品中CLZ和LRP的同时测定。
更新日期：2020-12-14
中文翻译：
使用具有不同受体相的双溶剂搅拌棒微萃取同时进行高效液相色谱紫外检测同时从人血浆中提取和分析氯氮平和劳拉西m
开发了一种新的双溶剂搅拌棒微萃取（DSSBME）设计，并与HPLC-UV结合使用，可从具有不同受体相的人血浆中同时提取氯氮平（CLZ）和劳拉西m（LRP）。将固定有有机萃取溶剂的两条短中空纤维用作从样品溶液中微萃取CLZ和LRP的溶剂棒。用用作搅拌器的订书钉固定溶剂棒。同时将目标分析物从样品溶液中选择性提取到其相应的溶剂棒中。优化了萃取参数，如有机溶剂类型，样品溶液的pH，受体相浓度，溶液中的盐掺入量，搅拌速率和萃取时间，以实现最佳萃取效果。-1和LRP为1.1 ng mL -1。CLZ的线性范围为1.3–1000.0 ng mL -1（ R 2 = 0.9991），LRP的线性范围为3.6–800.0 ng mL -1（ R 2 = 0.9993）。CLZ的提取回收率和富集因子分别为95.4％和343，LRP的提取回收率和富集因子分别为74.3％和263。最终，开发的方法成功地用于人血浆样品中CLZ和LRP的同时测定。
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