For end-stage lung disease, lung transplantation remains the only treatment but is limited by the availability of organs. Production of bioengineered lungs via recellularization is an alternative but is hindered by inadequate repopulation. We present a cell delivery method via the generation of negative pressure. Decellularized lungs were seeded with human bronchial epithelial cells using gravity-based perfusion or negative pressure (via air removal). After delivery, lungs were maintained in static conditions for 18 h, and cell surface coverage was qualitatively assessed using histology and analyzed by subjective scoring and an image analysis software. Negative pressure seeded lungs had higher cell surface coverage area, and this effect was maintained following 5 days of culture. Enhanced coverage via negative pressure cell delivery was also observed when vasculature seeded with endothelial cells. Our findings show that negative pressure cell delivery is a superior approach for the recellularization of the bioengineered lung.

中文翻译：

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负压细胞递送增强脱细胞肺的再细胞化

对于终末期肺病，肺移植仍然是唯一的治疗方法，但受到器官可用性的限制。通过再细胞化生产生物工程肺是一种替代方法，但受到再增殖不足的阻碍。我们提出了一种通过产生负压的细胞输送方法。使用基于重力的灌注或负压（通过空气去除），用人支气管上皮细胞接种脱细胞肺。分娩后，肺在静态条件下保持 18 小时，使用组织学定性评估细胞表面覆盖率，并通过主观评分和图像分析软件进行分析。负压接种肺具有更高的细胞表面覆盖面积，并且这种效果在培养 5 天后得以维持。当用内皮细胞接种脉管系统时，还观察到通过负压细胞递送增强的覆盖率。我们的研究结果表明，负压细胞递送是生物工程肺再细胞化的一种优越方法。