Organophosphorus flame retardants (OPFRs) have been extensively used as chemical additives in polymer based consumer products. Among them, Isopropylphenyl phosphate (IPPP) and tripropyl phosphate (TPP) are predominant, which have potential to cause neuro-toxic effects on non-target organisms. As behavior (swimming activity) response is the first adjustment due to neurotoxic stress on the fitness of fish. In this study, the quantified swimming activity of zebrafish (Danio rerio) under IPPP and TPP exposure in an online monitoring system was investigated to assess the neurotoxin effects under long-term exposure periods, no swimming anomalies were observed in the control group. Whereas, in the OPFR exposures ((treatment I: 5 μg/L and treatment II: 25 μg/L), a series of anomalies were identified. Hyperactivity was shown in IPPP treatment I group (5 μg/L), whereas zebrafish swimming activity was declined throughout the study period in IPPP treatment II (25 μg/L), and TPP groups (5 μg/L and 25 μg/L) when compared to the control group. Circadian rhythm was not affected in the present study. The results of the present study indicated that the fitness of test individuals was a valid biomarker for eco-toxicity assessment under unescapable conditions. Hypoactivity of zebrafish signified the neurotoxic effects of IPPP and TPP. A concentration based improvement in swimming activity was observed under recovery conditions, which suggested that recovery capacity along with toxicity responses could be a comprehensive non-invasive technique to assess the eco-toxicity of waterborne chemicals.

有机磷阻燃剂 (OPFR) 已广泛用作聚合物消费品中的化学添加剂。其中，磷酸异丙苯酯 (IPPP) 和磷酸三丙酯 (TPP) 占主导地位，它们有可能对非靶标生物造成神经毒性作用。由于行为（游泳活动）反应是由于神经毒性压力对鱼的健康造成的第一次调整。本研究对斑马鱼（Danio rerio) 在线监测系统中 IPPP 和 TPP 暴露下进行研究，以评估长期暴露期间的神经毒素影响，对照组未观察到游泳异常。然而，在 OPFR 暴露（（处理 I：5 μg/L 和处理 II：25 μg/L）中，发现了一系列异常。IPPP 处理 I 组（5 μg/L）显示多动，而斑马鱼游泳与对照组相比，IPPP 治疗 II（25 μg/L）和 TPP 组（5 μg/L 和 25 μg/L）在整个研究期间的活性下降。本研究中昼夜节律不受影响。本研究的结果表明，在不可避免的条件下，测试个体的适应性是生态毒性评估的有效生物标志物。斑马鱼的低活动性表明 IPPP 和 TPP 的神经毒性作用。