Background

In the present study, we hypothesized that feeding rats a high-fat diet negatively affects liver metabolism and function and disturbs the histology of some internal organs. We also postulated that there is a form of chromium whose administration alleviates the negative effects of a high-fat diet in rats.

Methods

To verify the hypotheses, we tested the effect of various forms of chrome (picolinate – Cr-Pic, Chromium(III)-methionine complex – Cr-Met, and chrome nanoparticles – Cr-NPs) applied in the recommended amount of 0.3 mg/kg of BW on growth parameters, body fat, liver metabolism and functional disorders, and histological parameters of selected internal organs in rats fed a standard (S) or high-fat diet (F). The experiment was conducted on 56 male outbred Wistar rats (Rattus norvegicus. Cmdb:WI) randomly divided into eight experimental groups. For eight weeks the rats received a standard or high-fat diet, without Cr or with Cr at 0.3 mg/kg diet in the form of Cr-Pic, Cr-Met or Cr-NPs.

Results and conclusion

The use of a F diet disrupted the lipid-carbohydrate profile, worsened liver metabolism and function, reduced the expression of hepatic PPAR-α and leaded to negative changes in the histological image of internal organs - liver, kidneys and pancreas. The 8-week use of an chromium supplement in a F diet, regardless of the form used, did not improve the ratio of fat tissue to lean tissue, worsened liver function and negatively affected on the histological image of the liver, kidneys and pancreas. However, the most negative changes in lipid-carbohydrate metabolism and liver functioning were observed with CrNPs supplementation.

中文翻译：

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高脂饮食补充各种形式的铬对大鼠身体成分、肝脏代谢和器官组织学的影响肝脏代谢和选定器官组织学中的Cr

背景

在本研究中，我们假设给大鼠喂食高脂饮食会对肝脏代谢和功能产生负面影响，并干扰一些内脏器官的组织学。我们还假设存在一种形式的铬，其给药可减轻大鼠高脂肪饮食的负面影响。

方法

为了验证假设，我们测试了各种形式的铬（吡啶甲酸 - Cr-Pic、铬（III）-蛋氨酸复合物 - Cr-Met 和铬纳米粒子 - Cr-NPs）的效果，推荐用量为 0.3 mg/公斤体重对喂食标准 (S) 或高脂肪饮食 (F) 的大鼠的生长参数、体脂肪、肝脏代谢和功能障碍以及选定内脏器官的组织学参数的影响。实验在56只雄性远交Wistar大鼠( Rattus norvegicus.Cmdb :WI)上进行，随机分为8个实验组。八周内，大鼠接受标准或高脂肪饮食，不含 Cr 或含 0.3 mg/kg Cr 的 Cr-Pic、Cr-Met 或 Cr-NPs 饮食。

结果与结论

使用 F 饮食会破坏脂质 - 碳水化合物谱，恶化肝脏代谢和功能，降低肝脏PPAR-α的表达，并导致内脏器官（肝脏、肾脏和胰腺）的组织学图像发生负面变化。在 F 饮食中使用铬补充剂 8 周，无论使用何种形式，都没有改善脂肪组织与瘦肉组织的比例，使肝功能恶化，并对肝脏、肾脏和胰腺的组织学图像产生负面影响。然而，在补充 CrNPs 的情况下，观察到脂质 - 碳水化合物代谢和肝功能的最负面变化。