The antifungal application of photodynamic therapy (PDT) has been widely explored. According to superficial nature of tinea capitis and the facility of application of light sources, the use of nanoencapsulated hypericin in P-123 associated with PDT (P123-Hy-PDT) has been a poweful tool to treat this pathology. Thus, the aim of this study was to evaluate the efficiency of P123-Hy-PDT against planktonic cells and in a murine model of dermatophytosis caused by Microsporum canis. In vitro antifungal susceptibility and in vivo efficiency tests were performed, including a skin toxicity assay, analysis of clinical signs by evaluating score, and photoacoustic spectroscopy. In addition, tissue analyses by histopathology and levels of pro-inflammatory cytokines, such as quantitative and qualitative antifungal assays, were employed. The in vitro assays demonstrated antifungal susceptibility with 6.25 and 12.5 μmol/L P123-Hy-PDI; these experiments are the first that have used this treatment of animals. P123-Hyp-mediated PDT showed neither skin nor biochemical alteration in vivo; it was safe for dermatophytosis treatment. Additionally, the treatment revealed rapid improvement in clinical signs at the site of infection after only three treatment sessions, with a clinical score confirmed by photoacoustic spectroscopy. The mycological reduction occurred after six treatment sessions, with a statistically significant decrease compared with untreated infected animals. These findings showed that P123-Hy-PDT restored tissue damage caused by infection, a phenomenon confirmed by histopathological analysis and proinflammatory cytokine levels. Our results reveal for the first time that P123-Hy-PDT is a promising treatment for tinea capitis and tinea corporis caused by M. canis, because it showed rapid clinical improvement and mycological reduction without causing toxicity.

光动力疗法（PDT）的抗真菌应用已被广泛探索。根据头癣的表面性质和光源的便利性，在与PDT相关的P-123（P123-Hy-PDT）中使用纳米胶囊化的金丝桃素是治疗这种病理的有效工具。因此，本研究的目的是评估P123-Hy-PDT对抗浮游细胞的效率以及在犬小孢子菌引起的皮肤癣菌病的小鼠模型中的作用。体外抗真菌药性和体内进行了效率测试，包括皮肤毒性测定，通过评估得分分析临床体征和光声光谱。另外，采用了通过组织病理学和促炎细胞因子水平的组织分析，例如定量和定性的抗真菌分析。在体外用6.25和12.5微摩尔/ L P123-HY-PDI测定证明抗真菌敏感性; 这些实验是首次使用这种动物处理方法的实验。P123-Hyp介导的PDT在体内未显示皮肤或生化改变; 对于皮肤癣菌病治疗是安全的。另外，该治疗仅在三个疗程后就显示出感染部位的临床症状迅速改善，并且通过光声光谱法证实了临床评分。六个疗程后发生真菌学降低，与未经治疗的感染动物相比，统计学上显着降低。这些发现表明，P123-Hy-PDT可恢复感染引起的组织损伤，这种现象已通过组织病理学分析和促炎细胞因子水平得到证实。我们的研究结果首次揭示了P123-Hy-PDT是一种有前途的治疗犬念珠菌引起的头癣和体癣的方法。，因为它显示出快速的临床改善和真菌学减轻而不会引起毒性。