Transcriptional re-programming in host and pathogen upon leaf and neck infection is an evolving area of research for the rice blast community. Analysis of in planta rice transcriptome in leaf and neck tissues revealed tissue-specific and infection-specific expression of rice and Magnaporthe oryzae genes in host and pathogen. The glycosyl hydrolase, isocitrate lyase, cupin domain containing protein, TF2, CMPG1, CHIT17 and OsCML14 genes were uniquely expressed in leaf infection. Genes like cytochrome P450, inhibitor I family protein, GSTU6, abscisic stress ripening, and cupin domain containing protein were up-regulated during neck infection. In our microRNA sequencing study, Osa-miR166n-3p was highly expressed in upon Magnaporthe leaf infection, whereas osa-miR1661-3p, osa-miR166n-3p and osa-miR159b were overexpressed in neck infection. Here we report several transcripts being targeted by up and down regulated microRNAs during infection. The putative genes expressed upon infection in leaf and neck could be used in understanding the dual-epidemics of blast disease.

叶和颈部感染后宿主和病原体的转录重编程是稻瘟病群落的一个不断发展的研究领域。对水稻叶片和颈部组织中植物转录组的分析揭示了水稻和稻瘟病菌基因在宿主和病原体中的组织特异性和感染特异性表达。糖基水解酶、异柠檬酸裂解酶、含有cupin结构域的蛋白、TF2、CMPG1、CHIT17和OsCML14基因在叶片侵染中唯一表达。在颈部感染期间，细胞色素 P450、抑制剂 I 家族蛋白、GSTU6、脱落应激成熟和含有蛋白质的 cupin 结构域等基因上调。在我们的 microRNA 测序研究中，Osa-miR166n-3p 在Magnaporthe上高度表达叶感染，而 osa-miR1661-3p、osa-miR166n-3p 和 osa-miR159b 在颈部感染中过表达。在这里，我们报告了在感染期间被上调和下调的 microRNA 靶向的几个转录物。在叶片和颈部感染时表达的推定基因可用于理解稻瘟病的双重流行。