Purpose

To develop and characterize a new type of plasma rich in growth factors (PRGF) membrane for patients in which immune system is involved in the disease etiology.

Methods

Blood from three healthy donors was collected to obtain the different fibrin membranes by PRGF technology. PRGF obtained volumes were activated and divided into two groups: PRGF membrane (mPRGF) obtained after incubation at 37 °C for 30 min (control); and is-mPRGF: mPRGF obtained after incubation for 30 min at 56 °C. The concentration of several growth factors, proteins, immunoglobulin E and the complement activity was determined in the different mPRGF. The proliferative potential of heat-inactivated mPRGF were assayed on keratocytes (HK) and conjunctival fibroblasts (HConF). In addition, morphological and physical features of the inactivated mPRGF were evaluated in contrast to the control mPRGF.

Results

Heat-inactivation of the mPRGF preserves the content of most of the growth factors involved in the ocular wound healing while reducing drastically the content of IgE and the complement activity. The heat-inactivated mPRGF conserve the morphological and physical characteristics of the fibrin meshwork in comparison with the control mPRGF. Furthermore, no significant differences were found in the biological activity of the control mPRGF regarding the heat-inactivated mPRGF (is-mPRGF) in any of both ocular cell types evaluated.

Conclusions

The heat-inactivation of the PRGF membranes (is-mPRGF) reduces drastically the content of IgE and complement activity while preserving the content of most of the proteins and morphogens involved in ocular wound healing. Furthermore, the morphological and physical features of the immunosafe mPRGF were also preserved after heat-inactivation.

中文翻译：

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源自富含生长因子技术血浆的个性化纤维蛋白膜的开发和优化

目的

为其中免疫系统参与疾病病因的患者开发和表征新型的富含生长因子（PRGF）膜的血浆。

方法

通过PRGF技术收集来自三名健康供体的血液，以获得不同的纤维蛋白膜。激活获得的PRGF的体积并将其分为两组：在37°C孵育30分钟后获得的PRGF膜（mPRGF）（对照）；将其分离。和is-mPRGF：在56℃下孵育30分钟后获得的mPRGF。在不同的mPRGF中测定几种生长因子，蛋白质，免疫球蛋白E和补体活性的浓度。在角膜细胞（HK）和结膜成纤维细胞（HConF）上测定热灭活的mPRGF的增殖潜力。另外，与对照mPRGF相比，评估了灭活的mPRGF的形态和物理特征。

结果

mPRGF的热失活保留了眼伤口愈合中涉及的大多数生长因子的含量，同时大大降低了IgE的含量和补体活性。与对照mPRGF相比，热灭活的mPRGF保留了纤维蛋白网的形态和物理特性。此外，在所评估的两种眼细胞类型中的任何一种中，关于热灭活的mPRGF（is-mPRGF），对照mPRGF的生物学活性均未发现显着差异。

结论

PRGF膜的热失活（is-mPRGF）大大降低了IgE的含量和补体活性，同时保留了涉及眼伤口愈合的大多数蛋白质和形态发生素的含量。此外，热灭活后还保留了免疫安全性mPRGF的形态和物理特征。